PXD033377 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cisplatin toxicity is counteracted by the activation of the p38/ATF-7 signaling pathway in post-mitotic C. elegans |
Description | Cisplatin kills proliferating cells via DNA damage but also has profound effects on post-mitotic cells in tumors, kidneys, and neurons. However, the effects of cisplatin on post-mitotic cells are still poorly understood. Among model systems, C. elegans adults are unique in having completely post-mitotic somatic tissues. The p38 MAPK pathway controls ROS detoxification via SKN-1/NRF and immune responses via ATF-7/ATF2. We show that p38 MAPK pathway mutants are sensitive to cisplatin, but while cisplatin exposure increases ROS levels, skn-1 mutants are resistant. Cisplatin exposure leads to phosphorylation of PMK-1/MAPK and ATF-7 and the IRE-1/TRF-1 signaling module functions upstream of the p38 MAPK pathway to activate signaling. We identify the response proteins whose increased abundance depends on IRE-1/p38 MAPK activity as well as cisplatin exposure. Four of these proteins are necessary for protection from cisplatin toxicity, which is characterized by necrotic death. We conclude that the p38 MAPK pathway-driven proteins are crucial for adult cisplatin resilience. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-03 |
AnnouncementXML | Submission_2024-10-03_06:37:49.524.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Proteomics Core Facility |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-23 15:16:48 | ID requested | |
1 | 2023-04-26 02:54:43 | announced | |
⏵ 2 | 2024-10-03 06:37:50 | announced | 2024-10-03: Updated project metadata. |
Publication List
10.1038/s41467-023-38568-5; |
Raj D, Kraish B, Martikainen J, Podraza-Farhanieh A, Kao G, Naredi P, Cisplatin toxicity is counteracted by the activation of the p38/ATF-7 signaling pathway in post-mitotic C. elegans. Nat Commun, 14(1):2886(2023) [pubmed] |
Keyword List
submitter keyword: innate immunity, drug sensitivity, cisplatin,p38 MAPK, Caenorhabditis elegans |
Contact List
Peter Naredi |
contact affiliation | Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE413 45 Gothenburg, Sweden |
contact email | peter.naredi@gu.se |
lab head | |
Proteomics Core Facility |
contact affiliation | SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg |
contact email | gupcf@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033377
- Label: PRIDE project
- Name: Cisplatin toxicity is counteracted by the activation of the p38/ATF-7 signaling pathway in post-mitotic C. elegans