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PXD033367

PXD033367 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePaleoproteomic profiling for identification of animal skin species in ancient Egyptian archaeological leather using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
DescriptionAncient protein analysis provides clues to human life and diseases from ancient times. Paleoproteomics has the potential to give a better understanding of the modes of fabrication of ancient materials, their composition, and pathways of degradation, as well as the development of animal fibers through domestication and breeding. Thus, this study aimed at providing guidance for choosing proteomics workflows to analyze leather samples and their capacity to distinguish between unknown archeological species. Here, we performed shotgun proteomics of archeological animal skin for the first time. The raw output data were analyzed using three different software (Proteome Discoverer, Protein Pilot, and Peptide Shaker) with their impeded algorithms. The study found that the best species identification percentage was obtained using protein piolet with protein database. Particularly prevalent and relatively high collagen expression suggests its resistance to degradation, despite the samples’ exposure to environmental and chemical alterations. The success of this case study indicates that further analyses could assist in reworking historical baseline data for putative identification of unknown archeological samples.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:23:43.033.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAli Anwar
SpeciesList scientific name: Camelus dromedarius papillomavirus 3; NCBI TaxID: 2714896; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600+
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-22 05:45:30ID requested
12023-01-04 05:02:19announced
22023-11-14 08:23:43announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MSMS,Archaeological, Proteomics
Contact List
Sameh Mohamed Magdeldin
contact affiliationProteomics and Metabolomics Research Program, Basic Research Department, Children’s Cancer Hospital, Cairo, Egypt.
contact emailsameh.magdeldin@57357.org
lab head
Ali Anwar
contact affiliationProteomics and metabolomics unit, Basic research department, Children's Cancer Hospital, 57357 Cairo, (CCHE-57357), Egypt
contact emailali.anwar@57357.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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