PXD033366

PXD033366 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Structure and mechanism of a novel cytomegaloviral DCAF mediating interferon antagonism
Description	Human cytomegalovirus (CMV) is a highly relevant pathogen, and its rodent counterparts serve as common infection models. Global proteome profiling of rat CMV (RCMV)-infected cells uncovered a pronounced loss of the transcription factor STAT2, which is crucial for interferon signalling. Deletion mutagenesis documented that STAT2 is targeted by the viral protein E27. Cellular analyses and in vitro reconstitution showed that E27 exploits host-derived Cullin4-RING ubiquitin ligases (CRL4) to induce poly-ubiquitylation and proteasomal degradation of STAT2. Cryo-electron microscopic structure determination revealed how E27 mimics molecular surface properties of cellular CRL4 substrate receptors to displace them from DDB1. Moreover, structure analyses solved the mechanism of STAT2 recruitment, and indicate that E27-binding disturbs the formation of active STAT2 complexes by occupying the IRF9-binding interface. Our results provide first structural insights into the cytomegalovirus-encoded interferon antagonism and establish an atomic model for STAT2 counteraction by CRL4 misappropriation, with important implications for viral immune evasion.
HostingRepository	jPOST
AnnounceDate	2022-12-15
AnnouncementXML	Submission_2022-12-14_07:00:04.666.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033366
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Graziadei
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification; unknown modification
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-22 03:43:27	ID requested
⏵ 1	2022-12-14 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: crosslinking mass spectrometry, XL-MS, DCAF, DDA1, DDB1, electron microscopy

submitter keyword: crosslinking mass spectrometry, XL-MS, DCAF, DDA1, DDB1, electron microscopy

Contact List

Juri Rappsilber
lab head
Andrea Graziadei
contact affiliation	Technische Universitaet Berlin
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001566/