PXD033364
PXD033364 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Targeted proteomics (parallel reaction monitoring) to quantify starch-biosynthetic proteins in yeast |
| Description | Heterologous protein expression in Saccharomyces cerevisiae is a powerful testbed to dissect the functions of plant starch biosynthetic enzymes and create diverse starch-like polymers. Here we modulated the expression each starch synthase together with a branching enzyme (derived from Arabidopsis thaliana) in a suite of yeast strains and quantified protein abundances using targeted proteomics. Specifically, we developed parallel reaction monitoring assays to quantify the Arabidopsis proteins SS1, SS2, SS3, SS4, BE2, BE3, ISA1, ISA2, among others (see Supplementary File 1, sheet 3) when expressed in yeast. We selected multiple surrogate peptides for each protein and characterized the assays with respect to their dynamic range (Supplementary Files 1 and 2). Data calibration was supported using crude stable isotope labeled (SIL) peptides. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2022-09-05 |
| AnnouncementXML | Submission_2022-09-05_13:44:52.185.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Barbara Pfister |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
| ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-04-21 11:58:08 | ID requested | |
| ⏵ 1 | 2022-09-05 13:44:52 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: starch, heterologous expression, starch synthase, branching enzyme, debranching enzyme, isoamylase |
Contact List
| Samuel C. Zeeman | |
|---|---|
| contact affiliation | ETH Zurich |
| contact email | szeeman@ethz.ch |
| lab head | |
| Barbara Pfister | |
| contact affiliation | Institute of Molecular Plant Biology, ETH Zurich |
| contact email | bpfister@ethz.ch |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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