PXD033348

PXD033348 is an original dataset announced via ProteomeXchange.

Title	A toolbox for systematic discovery of stable and transient protein interactors in baker’s yeast
Description	Identification of both stable and transient interactions is essential for understanding protein function and regulation. While assessing stable interactions is more straightforward, capturing transient ones is challenging. In recent years, sophisticated tools have emerged to improve transient interactor discovery, with many harnessing the power of evolved biotin ligases for proximity labelling. However, biotinylation-based methods have lagged behind in the model eukaryote, Saccharomyces cerevisiae, possibly due to the presence of several abundant, endogenously biotinylated proteins. In this study, we optimised robust biotin-ligation methodologies in yeast and increased their sensitivity by creating a bespoke technique for downregulating endogenous biotinylation which we term ABOLISH (Auxin-induced BiOtin LIgase diminiSHing). We used the endoplasmic reticulum insertase complex (EMC) to demonstrate our approaches and uncover new substrates. To make these tools available for systematic probing of both stable and transient interactions, we generated five full-genome collections of strains in which every yeast protein is tagged with each of the tested biotinylation machineries; some on the background of the ABOLISH system. This comprehensive toolkit enables functional interactomics of the entire yeast proteome.
HostingRepository	PRIDE
AnnounceDate	2023-02-01
AnnouncementXML	Submission_2023-02-01_10:04:43.899.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MeitalKupervaser
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-21 06:49:32	ID requested
⏵ 1	2023-02-01 10:04:44	announced

Dataset with its publication pending

submitter keyword: substrate discovery, TurboID, yeast libraries,interaction profiling

MayaSchuldiner
contact affiliation	Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel
contact email	maya.schuldiner@weizmann.ac.il
lab head
MeitalKupervaser
contact affiliation	Weizmann Institute of Science
contact email	meital.kupervaser@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD033348

PRIDE project URI

• PRIDE
  1. PXD033348
     1. Label: PRIDE project
     2. Name: A toolbox for systematic discovery of stable and transient protein interactors in baker’s yeast