PXD033337

PXD033337 is an original dataset announced via ProteomeXchange.

Title	A truncated and catalytically inactive form of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression
Description	KDM5B histone demethylase is overexpressed in many cancers with an ambivalent role in oncogenesis which depends on the specific contest. A putative explanation of this ambivalence could be represented by the expression pattern of different protein isoforms with different functional roles which could be present at different levels in different cancer cell lines. We show here that one of these isoforms (NTT) accumulates in breast cancer cell lines up to 50-60% of the total, due to a remarkable protein stability relative to the canonical PLU-1 isoform which shows a much faster turnover. Mass Spectrometry (MS) profiling of histone post-translational modifications showed that overexpression of this isoform in MCF7 cells leads to an increase in H3K4 trimethylation. We discuss the relevance of this finding at the light of the hypothesis that KDM5B may possess regulatory roles independent of its catalytic activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:55:12.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giulia Robusti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; trimethylated residue; dimethylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-21 06:38:30	ID requested
1	2023-07-20 11:03:54	announced
2	2023-11-14 09:03:05	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:55:13	announced	2024-10-22: Updated project metadata.

10.1038/s41417-022-00584-w;

Di Nisio E, Licursi V, Mannironi C, Buglioni V, Paiardini A, Robusti G, Noberini R, Bonaldi T, Negri R, A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression. Cancer Gene Ther, 30(6):822-832(2023) [pubmed]

submitter keyword: Histone PTMs, epigenetics, HMEs

Tiziana Bonaldi
contact affiliation	Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Milan, Italy Department of Oncology and Hematology-Oncology, University of Milan, Milan, Italy
contact email	tiziana.bonaldi@ieo.it
lab head
Giulia Robusti
contact affiliation	European Institute of Oncology
contact email	giulia.robusti@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033337

PRIDE project URI

• PRIDE
  1. PXD033337
     1. Label: PRIDE project
     2. Name: A truncated and catalytically inactive form of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression