PXD033331

PXD033331 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of rainbow trout (Oncorhynchus mykiss) exposed to microplastics
Description	The pollution of the environment with microplastics has been recognized as an emerging threat worldwide. Due to an exponential increase in production of plastic over the last eight decades and its longevity in the environment, accumulating amounts of microplastic are polluting rivers, lakes and the ocean. Their entry pathways are diverse and still only incompletely understood. Since microplastics are usually defined smaller than 5 mm, it can be ingested by a wide range of aquatic organisms including teleost fish. There are different approaches to study the detrimental effects of pollutants on aquatic organisms. On the one hand, generic baseline parameters such as growth and mortality are regularly considered, often accompanied by established stress parameters such as cortisol, heat shock proteins or lipid oxidation. The conflicting findings to date suggest that these parameters might not be sensitive enough to indicate the physiological effects of environmentally relevant microplastic concentrations. For this reason, more sophisticated biological approaches could provide new insights into whether and how microplastics harm fish. To date, proteomic approaches have been used only sporadically when investigating the effects of microplastic exposure on aquatic organisms. So far, this approach has not been used to address potential microplastic impacts in fish. In the present study, a proteomic approach was trialed alongside established methods in an investigation of fish experiencing long-term exposure to environmentally relevant concentrations of microplastics. Two groups of rainbow trout (Oncorhynchus mykiss were exposed to microplastic concentrations and sizes currently encountered in wild fish and an increased concentration, expected to occur in the near future. These groups where compared to a control group maintained in MP free conditions. Five fish of each treatment were sampled at three time points (week 1, week 4, week 17). The experiments were performed in triplicates, resulting in 45 samples used in the proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:31:49.694.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samuel Roch
SpeciesList	scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-21 03:50:33	ID requested
1	2022-08-08 06:25:51	announced
⏵ 2	2023-11-14 08:31:50	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Q Exactive HF-X, liver, Oncorhynchus mykiss,microplastics, rainbow trout

Samuel Roch
contact affiliation	Agricultural Center Baden-Württemberg, Fisheries Research Station, Germany
contact email	samuel.roch@lazbw.bwl.de
lab head
Samuel Roch
contact affiliation	Fisheries Research Station Baden-Württemberg
contact email	samuel.roch@lazbw.bwl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033331

PRIDE project URI

• PRIDE
  1. PXD033331
     1. Label: PRIDE project
     2. Name: Proteomic analysis of rainbow trout (Oncorhynchus mykiss) exposed to microplastics