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PXD033301

PXD033301 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title4E-BP1 counteracts human stem cell senescence via maintaining mitochondrial homeostasis
DescriptionAlthough the mTOR-4E-BP1 signaling pathway is implicated in aging and aging-related disorders, the role of 4E-BP1 in regulating human stem cell homeostasis remains largely unknown. Here, we report that the expression of 4E-BP1 decreases along with the senescence of human mesenchymal stem cells (hMSCs). Genetic inactivation of 4E-BP1 in hMSCs accelerates cellular senescence, compromises mitochondrial respiration and increases mitochondrial ROS production. Mechanistically, the absence of 4E-BP1 destabilizes proteins in mitochondrial respiration complexes, especially several key subunits of the complex III including UQCRC2. Ectopic expression of 4E-BP1 attenuates mitochondrial abnormalities and alleviates cellular senescence in 4E-BP1-deficient hMSCs as well as in physiologically aged hMSCs. These findings together demonstrate that 4E-BP1 functions as a geroprotector to alleviate human stem cell senescence and maintain mitochondrial homeostasis, particularly for the mitochondrial respiration complex III and provide a new potential target to counteract human stem cell senescence.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:46:48.758.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterji zhao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-19 06:54:48ID requested
12023-05-10 13:39:10announced
22023-11-14 08:46:50announced2023-11-14: Updated project metadata.
Publication List
He Y, Ji Q, Wu Z, Cai Y, Yin J, Zhang Y, Zhang S, Liu X, Zhang W, Liu GH, Wang S, Song M, Qu J, 4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis. Protein Cell, 14(3):202-216(2023) [pubmed]
Keyword List
submitter keyword: EIF4EBP1;mitochondria;oxidative phosphorylation
Contact List
Qianzhao Ji
contact affiliation+8617812007228
contact emailjiqianzhaonotold@outlook.com
lab head
ji zhao
contact affiliation17812007228
contact emailjiqianzhaonotold@outlook.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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