PXD033301

PXD033301 is an original dataset announced via ProteomeXchange.

Title	4E-BP1 counteracts human stem cell senescence via maintaining mitochondrial homeostasis
Description	Although the mTOR-4E-BP1 signaling pathway is implicated in aging and aging-related disorders, the role of 4E-BP1 in regulating human stem cell homeostasis remains largely unknown. Here, we report that the expression of 4E-BP1 decreases along with the senescence of human mesenchymal stem cells (hMSCs). Genetic inactivation of 4E-BP1 in hMSCs accelerates cellular senescence, compromises mitochondrial respiration and increases mitochondrial ROS production. Mechanistically, the absence of 4E-BP1 destabilizes proteins in mitochondrial respiration complexes, especially several key subunits of the complex III including UQCRC2. Ectopic expression of 4E-BP1 attenuates mitochondrial abnormalities and alleviates cellular senescence in 4E-BP1-deficient hMSCs as well as in physiologically aged hMSCs. These findings together demonstrate that 4E-BP1 functions as a geroprotector to alleviate human stem cell senescence and maintain mitochondrial homeostasis, particularly for the mitochondrial respiration complex III and provide a new potential target to counteract human stem cell senescence.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:48.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ji zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-19 06:54:48	ID requested
1	2023-05-10 13:39:10	announced
⏵ 2	2023-11-14 08:46:50	announced	2023-11-14: Updated project metadata.

He Y, Ji Q, Wu Z, Cai Y, Yin J, Zhang Y, Zhang S, Liu X, Zhang W, Liu GH, Wang S, Song M, Qu J, 4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis. Protein Cell, 14(3):202-216(2023) [pubmed]

submitter keyword: EIF4EBP1;mitochondria;oxidative phosphorylation

Qianzhao Ji
contact affiliation	+8617812007228
contact email	jiqianzhaonotold@outlook.com
lab head
ji zhao
contact affiliation	17812007228
contact email	jiqianzhaonotold@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033301
     1. Label: PRIDE project
     2. Name: 4E-BP1 counteracts human stem cell senescence via maintaining mitochondrial homeostasis