PXD033292

PXD033292 is an original dataset announced via ProteomeXchange.

Title	Diazirine photo-immobilisation for target profiling of natural products and co-factors: pulldown data
Description	Finding the targets of natural products is of key importance in both chemical biology and drug discovery, and deconvolution of cofactor interactomes contribute to the functional annotation of the proteome. Identifying the proteins that underlie natural compound activity in phenotypic screens help to validate the respective targets and, potentially, expand the druggable proteome. Here, we present a generally applicable protocol for the photoactivated immobilisation of unmodified and microgram quantities of natural products on diazirine-decorated beads and their use for systematic affinity-based proteome profiling. We show that amongst 31 molecules of very diverse reported activity and biosynthetic origin, 25 could indeed be immobilised. Dose-response competition binding experiments using lysates of human or bacterial cells followed by quantitative mass spectrometry recapitulated <100 µM targets of 9 molecules. Among them, immobilisation of coenzyme A produced a tool to interrogate proteins containing a HotDog domain. Surprisingly, immobilisation of the cofactor flavin adenine dinucleotide (FAD) led to the identification of nanomolar interactions with dozens of RNA-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2022-10-06
AnnouncementXML	Submission_2022-10-06_06:43:00.275.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Polina Prokofeva
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-19 03:16:42	ID requested
⏵ 1	2022-10-06 06:43:00	announced

Dataset with its publication pending

submitter keyword: pulldowns, natural products targets, natural products profiling, photo-immobilisation

Prof. Dr. Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics Technical University of Munich Emil Erlenmeyer Forum 5 85354 Freising Germany
contact email	kuster@tum.de
lab head
Polina Prokofeva
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Emil-Erlenmeyer-Forum 5, 85354 Freising, Germany
contact email	polina.prokofeva@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033292

PRIDE project URI

• PRIDE
  1. PXD033292
     1. Label: PRIDE project
     2. Name: Diazirine photo-immobilisation for target profiling of natural products and co-factors: pulldown data