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PXD033289

PXD033289 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts
DescriptionZika virus (ZIKV) infection at the maternal-placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) protein, mRNA, and miRNA cargo, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model.
HostingRepositoryPRIDE
AnnounceDate2022-05-20
AnnouncementXMLSubmission_2022-05-20_09:43:55.068.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD033289
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLindsey Block
SpeciesList scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-19 03:08:02ID requested
12022-05-20 09:43:55announced
Publication List
Block LN, Schmidt JK, Keuler NS, McKeon MC, Bowman BD, Wiepz GJ, Golos TG, Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts. Sci Rep, 12(1):7348(2022) [pubmed]
Keyword List
submitter keyword: trophoblast, placenta, zika virus, exosome, extracellular vesicle
Contact List
Thaddeus Golos
contact affiliationUniversity of Wiscosin Madison Wisconsin National Primate Research Center
contact emailgolos@primate.wisc.edu
lab head
Lindsey Block
contact affiliationUW-Madison UPenn
contact emaillindsey.block@pennmedicine.upenn.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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