PXD033289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts |
Description | Zika virus (ZIKV) infection at the maternal-placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) protein, mRNA, and miRNA cargo, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_09:43:55.068.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD033289 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Lindsey Block |
SpeciesList | scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-19 03:08:02 | ID requested | |
⏵ 1 | 2022-05-20 09:43:55 | announced | |
Publication List
Block LN, Schmidt JK, Keuler NS, McKeon MC, Bowman BD, Wiepz GJ, Golos TG, Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts. Sci Rep, 12(1):7348(2022) [pubmed] |
Keyword List
submitter keyword: trophoblast, placenta, zika virus, exosome, extracellular vesicle |
Contact List
Thaddeus Golos |
contact affiliation | University of Wiscosin Madison Wisconsin National Primate Research Center |
contact email | golos@primate.wisc.edu |
lab head | |
Lindsey Block |
contact affiliation | UW-Madison UPenn |
contact email | lindsey.block@pennmedicine.upenn.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033289
- Label: PRIDE project
- Name: Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts