PXD033289

PXD033289 is an original dataset announced via ProteomeXchange.

Title	Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts
Description	Zika virus (ZIKV) infection at the maternal-placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) protein, mRNA, and miRNA cargo, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_09:43:55.068.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033289
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lindsey Block
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-19 03:08:02	ID requested
⏵ 1	2022-05-20 09:43:55	announced

Block LN, Schmidt JK, Keuler NS, McKeon MC, Bowman BD, Wiepz GJ, Golos TG, Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts. Sci Rep, 12(1):7348(2022) [pubmed]

submitter keyword: trophoblast, placenta, zika virus, exosome, extracellular vesicle

Thaddeus Golos
contact affiliation	University of Wiscosin Madison Wisconsin National Primate Research Center
contact email	golos@primate.wisc.edu
lab head
Lindsey Block
contact affiliation	UW-Madison UPenn
contact email	lindsey.block@pennmedicine.upenn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033289
     1. Label: PRIDE project
     2. Name: Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts