PXD033244

PXD033244 is an original dataset announced via ProteomeXchange.

Title	Calcium-dependent proximal biotinylation via Cal-ID
Description	Calcium ions serve as key intracellular signals. Local, transient increases in calcium concentrations can activate calcium sensor proteins that in turn trigger downstream effectors. In neurons, such calcium transients trigger pre-synaptic vesicle release and mediate post-synaptic plasticity. It is challenging to capture the molecular events associated with these localized and ephemeral calcium signals, however. Here we report the development of an engineered biotin ligase that combines the power of genetically encoded calcium indicators with protein proximity labeling. The enzyme, Cal-ID, biotinylates nearby proteins in response to elevated local calcium levels. The biotinylated proteins can be visualized by microscopy and identified via mass spectrometry. Cal-ID mass spectrometry applied to HEK293T cells identified cell cycle-dependent calcium signaling microdomains at centrosomes. Our results from mouse primary neurons indicated active calcium signaling near calcium extrusion sites on the plasma membrane. Therefore, we propose Cal-ID as a biochemical recorder of calcium signaling in living cells.
HostingRepository	PRIDE
AnnounceDate	2024-02-04
AnnouncementXML	Submission_2024-02-04_04:02:37.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J Wren Kim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 05:30:12	ID requested
⏵ 1	2024-02-04 04:02:37	announced

Dataset with its publication pending

submitter keyword: Cal-ID

Nicholas Ingolia
contact affiliation	UC Berkeley
contact email	ingolia@berkeley.edu
lab head
J Wren Kim
contact affiliation	UC Berkeley
contact email	jwrenkim@berkeley.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033244
     1. Label: PRIDE project
     2. Name: Calcium-dependent proximal biotinylation via Cal-ID