PXD033240

PXD033240 is an original dataset announced via ProteomeXchange.

Title	The membrane-intrinsic Zn-metalloproteinase FtsH4 does not associate with FtsH1, FtsH2 or FtsH3 but does interact with the uncharacterized protein Sll1106
Description	FtsH Zn-metalloproteinases are multimeric, membrane-intrinsic and universally conserved in bacteria, chloroplasts and mitochondria. FtsHs function in protein turnover and processing: activities that are essential in maintaining cellular homeostasis. In phototrophic organisms, FtsHs are additionally involved in the biogenesis and repair of the photosystem complexes. The cyanobacterium Synechocystis synthesizes 4 homologous FtsHs: FtsH1, 2, 3, and 4. While the first 3 are known to function as hetero-oligomers FtsH1/3 and FtsH2/3, interactions involving FtsH4 are not fully characterized. Here, we determined the relative amounts of the 4 FtsHs in wild-type Synechocystis thylakoid membranes using the iBAQ method. In conjunction with FLAG-affinity pulldown experiments, FtsH4 was found not to associate with the other FtsHs. However, the uncharacterized protein Sll1106 was identified as an FtsH interaction partner.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:53.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. PCC 6803 substr. GT-I; NCBI TaxID: 1080228;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 05:22:46	ID requested
1	2023-01-04 04:35:41	announced
⏵ 2	2023-11-14 08:55:54	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: biogenesis, cyanobacteria, Sll1106, FtsH,Synechocystis, FLAG-tag, photosystem, repair

Josef Komenda
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences, Třeboň, Czech Republic
contact email	komenda@alga.cz
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033240
     1. Label: PRIDE project
     2. Name: The membrane-intrinsic Zn-metalloproteinase FtsH4 does not associate with FtsH1, FtsH2 or FtsH3 but does interact with the uncharacterized protein Sll1106