PXD033237

PXD033237 is an original dataset announced via ProteomeXchange.

Title	Perchlorate-Specific Proteomic Stress Responses of Debaryomyces hansenii Could Enable Microbial Survival in Martian Brines
Description	If life exists on Mars, it would face several challenges including the presence of perchlorates, which destabilize biomacromolecules by inducing chaotropic stress. However, little is known about perchlorate toxicity for microorganism on the cellular level. Here we present a proteomic investigation on the perchlorate-specific stress responses of the halotolerant yeast Debaryomyces hansenii and compare these to generally known salt stress adaptations. We found that the responses to NaCl and NaClO4-induced stresses share many common metabolic features, e.g., signaling pathways, elevated energy metabolism, or osmolyte biosynthesis. However, several new perchlorate-specific stress responses could be identified, such as protein glycosylation and cell wall remodulations, presumably in order to stabilize protein structures and the cell envelope. These stress responses would also be relevant for life on Mars, which - given the environmental conditions - likely developed chaotropic defense strategies such as stabilized confirmations of biomacromolecules and the formation of cell clusters.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:34.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Doellinger
SpeciesList	scientific name: Debaryomyces hansenii; NCBI TaxID: 4959;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 05:18:51	ID requested
1	2023-06-06 01:39:11	announced
⏵ 2	2023-11-14 08:48:37	announced	2023-11-14: Updated project metadata.

Heinz J, Doellinger J, Maus D, Schneider A, Lasch P, Grossart HP, Schulze-Makuch D, Perchlorate-specific proteomic stress responses of Debaryomyces hansenii could enable microbial survival in Martian brines. Environ Microbiol, 24(11):5051-5065(2022) [pubmed]

submitter keyword: Debaryomyces hansenii, astrobiology, mars, martian brines, perchlorate, stress response

Peter Lasch
contact affiliation	ZBS6, Proteomics and Spectroscopy, Robert Koch-Institute, Germany, Berlin
contact email	LaschP@rki.de
lab head
Joerg Doellinger
contact affiliation	Proteomics and Spectroscopy (ZBS6), Robert Koch-Institute
contact email	Doellingerj@rki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD033237

PRIDE project URI

• PRIDE
  1. PXD033237
     1. Label: PRIDE project
     2. Name: Perchlorate-Specific Proteomic Stress Responses of Debaryomyces hansenii Could Enable Microbial Survival in Martian Brines