PXD033236

PXD033236 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of tumor tissue-derived extracellular vesicles in colon cancer
Description	Colon cancer is one of the most commonly occurring tumors among both women and men, and over the past decades the incidence has been on the rise. As such, the need for biomarker identification as well as an understanding of the underlying disease mechanism has never been greater. Extracellular vesicles are integral mediators of cell-to-cell communication and offer a unique opportunity to study the machinery that drives disease progression, and they also function as vectors for potential biomarkers. Tumor tissue and healthy mucosal tissue from the colons of ten patients were used to isolate tissue-resident EVs that were subsequently subjected to global quantitative proteomic analysis through LC-MS/MS. In total, more than 2000 proteins were identified, with most of the common EV markers being among them. Bioinformatics revealed a clear underrepresentation of proteins involved in energy production and cellular adhesion in tumor EVs, while proteins involved in protein biosynthesis were overrepresented. Additionally, 53 membrane proteins were found to be significantly upregulated in tumor EVs. Among them were several proteins with enzymatic functions that degrade the extracellular matrix, and two of these, FAP and TMEM2, as well as the ephrin receptor EPHB3, were validated and found to be consistent with the global quantitative results. These stark differences in the proteomes between healthy and cancerous tissue emphasize the importance of the interstitial vesicle secretome as a major player of disease development.
HostingRepository	PRIDE
AnnounceDate	2026-06-26
AnnouncementXML	Submission_2026-06-25_23:41:12.607.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 05:18:37	ID requested
⏵ 1	2026-06-25 23:41:14	announced

Cvjetkovic A, Karimi N, Crescitelli R, Thorsell A, Taflin H, L, ä, sser C, L, ö, tvall J, Proteomic profiling of tumour tissue-derived extracellular vesicles in colon cancer. J Extracell Biol, 3(2):e127(2024) [pubmed]

10.1002/jex2.127;

submitter keyword: interstitial vesicles, Extracellular Vesicles,Colorectal cancer, Proteomics, Exosomes, Biomarkers, tumor microenvironment

Aleksander Cvjetkovic
contact affiliation	Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	aleksanderc@chem.ku.dk
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD033236

PRIDE project URI

• PRIDE
  1. PXD033236
     1. Label: PRIDE project
     2. Name: Proteomic profiling of tumor tissue-derived extracellular vesicles in colon cancer