PXD033233

PXD033233 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The novel role of hnRNP UL1 in rDNA transcription and rDNA repair in human cell nucleoli
Description	hnRNP UL1 plays an important function in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli. Revealing its function, we figured out that hnRNP UL1 stimulates rDNA gene transcription and may be involved in the transport of the proteins between the nucleolus and the nucleoplasm. Moreover, we observed that cells with hnRNP UL1 silencing are more sensitive to DNA damage, suggesting its role in rDNA repair pathways and nucleolar genome integrity. Indeed, we confirmed that hnRNP UL1 interacts with yH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in repairing of DNA damages.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:05.788.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033233
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 04:49:27	ID requested
1	2022-10-14 20:13:52	announced
⏵ 2	2023-11-14 08:29:06	announced	2023-11-14: Updated project metadata.

Publication List

Cichocka M, Karlik A, Plewka P, Gawade K, St, ę, pie, ń A, Ś, wiergiel P, Gadgil A, Raczy, ń, ska KD, The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci, 18(13):4809-4823(2022) [pubmed]
10.6019/PXD033233;

Cichocka M, Karlik A, Plewka P, Gawade K, St, ę, pie, ń A, Ś, wiergiel P, Gadgil A, Raczy, ń, ska KD, The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci, 18(13):4809-4823(2022) [pubmed]

10.6019/PXD033233;

Keyword List

submitter keyword: LC-MSMS, nucleolus,human

submitter keyword: LC-MSMS, nucleolus,human

Contact List

Marlena Cichocka
contact affiliation	Department of Gene Expression, Laboratory of RNA Processing, Institute of Molecular Biology and Biotechnology, Faculty of Biology and Center for Advanced Technology, Adam Mickiewicz University, Poland
contact email	m.cichocka1992@gmail.com
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033233

PRIDE project URI

Repository Record List

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