PXD033229 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ANP32e binds histone H2A.Z in a cell cycle-dependent manner and regulates its protein stability in the cytoplasm |
Description | ANP32e, a chaperone of H2A.Z, is receiving increasing attention because of its link to cancer growth and progression. An unanswered question is whether ANP32e regulates H2A.Z dynamics during the cell cycle; if so, this could have clear implications for the proliferation of cancer cells. Using the human U2OS cancer cell line model system, we have confirmed that ANP32e regulates the growth of these cells. ANP32e preferentially interacts with H2A.Z during G1 phase of the cell cycle. Unexpectedly, however, ANP32e does not mediate the removal of H2A.Z from chromatin, is not a stable component of the p400 remodeling complex, and is not strongly associated with chromatin. Instead, most ANP32e is in the cytoplasm. Here, ANP32e preferentially interacts with H2A.Z in G1 phase in response to an increase in H2A.Z protein abundance and regulates its protein stability. This G1-specific interaction between ANP32e and H2A.Z is also observed in the nucleoplasm but is unrelated to any change in H2A.Z abundance. Collectively, these results challenge the idea that ANP32e is involved in regulating the abundance of H2A.Z in chromatin as part of a chromatin remodeling complex. Rather, we propose that ANP32e acts as a molecular chaperone that maintains the soluble pool of H2A.Z by regulating its protein stability and acting as a buffer in response to cell cycle-dependent changes in H2A.Z abundance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:41:28.537.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gene Hart-Smith |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-14 04:35:11 | ID requested | |
1 | 2024-05-22 23:45:26 | announced | |
⏵ 2 | 2024-10-22 06:41:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dijkwel Y, Hart-Smith G, Kurscheid S, Tremethick DJ, ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm. Mol Cell Biol, 44(2):72-85(2024) [pubmed] |
10.1080/10985549.2024.2319731; |
Keyword List
submitter keyword: protein stability, epigenetics, chromatin remodeling,ANP32e, chaperone, H2A.Z, cell cycle, chromatin |
Contact List
David J Tremethick |
contact affiliation | The John Curtin School of Medical Research, The Australian National University, Canberra, Australia |
contact email | david.tremethick@anu.edu.au |
lab head | |
Gene Hart-Smith |
contact affiliation | Macquarie University |
contact email | gene.hart-smith@mq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033229
- Label: PRIDE project
- Name: ANP32e binds histone H2A.Z in a cell cycle-dependent manner and regulates its protein stability in the cytoplasm