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PXD033229

PXD033229 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleANP32e binds histone H2A.Z in a cell cycle-dependent manner and regulates its protein stability in the cytoplasm
DescriptionANP32e, a chaperone of H2A.Z, is receiving increasing attention because of its link to cancer growth and progression. An unanswered question is whether ANP32e regulates H2A.Z dynamics during the cell cycle; if so, this could have clear implications for the proliferation of cancer cells. Using the human U2OS cancer cell line model system, we have confirmed that ANP32e regulates the growth of these cells. ANP32e preferentially interacts with H2A.Z during G1 phase of the cell cycle. Unexpectedly, however, ANP32e does not mediate the removal of H2A.Z from chromatin, is not a stable component of the p400 remodeling complex, and is not strongly associated with chromatin. Instead, most ANP32e is in the cytoplasm. Here, ANP32e preferentially interacts with H2A.Z in G1 phase in response to an increase in H2A.Z protein abundance and regulates its protein stability. This G1-specific interaction between ANP32e and H2A.Z is also observed in the nucleoplasm but is unrelated to any change in H2A.Z abundance. Collectively, these results challenge the idea that ANP32e is involved in regulating the abundance of H2A.Z in chromatin as part of a chromatin remodeling complex. Rather, we propose that ANP32e acts as a molecular chaperone that maintains the soluble pool of H2A.Z by regulating its protein stability and acting as a buffer in response to cell cycle-dependent changes in H2A.Z abundance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:41:28.537.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGene Hart-Smith
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-14 04:35:11ID requested
12024-05-22 23:45:26announced
22024-10-22 06:41:34announced2024-10-22: Updated project metadata.
Publication List
Dijkwel Y, Hart-Smith G, Kurscheid S, Tremethick DJ, ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm. Mol Cell Biol, 44(2):72-85(2024) [pubmed]
10.1080/10985549.2024.2319731;
Keyword List
submitter keyword: protein stability, epigenetics, chromatin remodeling,ANP32e, chaperone, H2A.Z, cell cycle, chromatin
Contact List
David J Tremethick
contact affiliationThe John Curtin School of Medical Research, The Australian National University, Canberra, Australia
contact emaildavid.tremethick@anu.edu.au
lab head
Gene Hart-Smith
contact affiliationMacquarie University
contact emailgene.hart-smith@mq.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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