PXD033224

PXD033224 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of Arabidopsis root in response to cellooligomers (cellotriose)
Description	Cellulose is the major polysaccharide in the primary and secondary cell wall. Its breakdown generates short-chain cellooligomers (COs) which induce Ca2+-dependent cell wall integrity (CWI) responses. Recently, a mutant (cork1) which did not respond to COs was identified. To find out the early phosphorylation events following CO perception, roots of the segregated wild-type and homozygous mutants (F2 generation from cork1-2 X AeqWT) were treated with 10 µM cellotriose or water for 0, 5 and 15 minutes. Phosphoproteome analyses identified early targets involved in signaling, the endoplasmatic recticulum/Golgi secretory pathway, cell wall repair and immune responses. The result identified protein targets of the novel CO-CORK1 pathway and shine light on the role of COs in CWI maintenance.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:21.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 04:24:12	ID requested
1	2022-09-24 03:35:39	announced
⏵ 2	2023-11-14 08:55:22	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Phosphoproteomics,Cellooligomer, Cellotriose, Malectin, Cell wall integrity

Axel A. Brakhage
contact affiliation	Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033224
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of Arabidopsis root in response to cellooligomers (cellotriose)