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PXD033207

PXD033207 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLaser Capture Microdissection of Normal Kidney Biopsy Glomeruli and Tubulointerstitium Characterized by LC-MS/MS
DescriptionThe Kidney Precision Medicine Project (KPMP) is funded by the NIDDK for "the purpose of understanding and finding new ways to treat chronic kidney disease and acute kidney injury. " To accomplish this goal, the KPMP chose several technologies to successfully deliver their goals. One of the technologies chosen was using a combination of laser capture microdissection (LCM) in combination with LC -MS /MS to characterize quantitative protein changes in the diseased kidney. The data presented here is from the initial pilot study using normal kidney biopsies for proof of principal. Samples analyzed were either LCM collected glomeruli or tubulointerstitium. Protein was retrieved from the LCM collected tissue, proteins were trypsinized and samples run on the fusion orbitrap.
HostingRepositoryMassIVE
AnnounceDate2022-04-15
AnnouncementXMLSubmission_2022-04-15_08:17:46.507.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDr. Miranda Gardner
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListL-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-13 09:05:26ID requested
12022-04-15 08:17:46announced
Publication List
no publication
Keyword List
submitter keyword: Laser Capture Microdissection, Normal Kidney Glomeruli , Normal Kidney Tubulointerstitium, Spectral Counts, Thermo Orbitrap Eclipse, Bottom-up Proteomics
Contact List
Samir Parikh
contact affiliationThe Ohio State University Wexner Medical Center
contact emailsamir.parikh@osumc.edu
lab head
Dr. Miranda Gardner
contact affiliationThe Ohio State University
contact emailgardner.207@osu.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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