PXD033194

PXD033194 is an original dataset announced via ProteomeXchange.

Title	Cardiac tissue remodeling as a new marker to predict cardiac dysfunction induced by radiotherapy.
Description	The contribution of radiotherapy, per se, in late cardiotoxicity remains controversial. An experimental model was developed, in which rat hearts were exposed to a range of ionizing radiation doses, to clarify its impact on the development of early cardiac dysfunction. Rat’s hearts were exposed to daily doses of 0.04, 0.3 and 1.2 Gy, for 23 days, achieving cumulative doses of 0.92, 6.9 and 27.6 Gy, respectively. Myocardial deformation, assessed by global longitudinal strain, was impaired (a relative percentage reduction of > 15% from baseline) in a dose-dependent manner. Moreover, by scanning electron microscopy, the microvascular density in the cardiac apex was significantly decreased exclusively at 27.6 Gy dosage. Prior to GLS impairment detection, several tools (qRT-PCR, mass spectrometry and western blot) were used to assess cardiac tissue remodeling. The number/expression of several genes, proteins and KEGG pathways, related to inflammation, fibrosis and cardiac muscle contraction, were found to be differently expressed in the cardiac tissue, according to the cumulative dose. Subclinical cardiac dysfunction occurred in a dose-dependent manner as detected by cardiac tissue remodeling, a predictor of the severity of global longitudinal strain impairment. Moreover, there is no dose threshold below which no myocardial deformation impairment was detected. These findings may contribute to i) develop new markers and explore non-invasive magnetic resonance imaging to assess cardiac tissue remodeling as an early predictor of cardiac dysfunction; ii) improve radiation-based diagnostic and therapeutic cardiac procedures; iii) highlight the need for personalized clinical approaches.
HostingRepository	PRIDE
AnnounceDate	2022-09-08
AnnouncementXML	Submission_2022-09-08_11:20:45.372.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033194
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-13 02:33:36	ID requested
⏵ 1	2022-09-08 11:20:46	announced

Ribeiro S, Sim, õ, es AR, Rocha F, Vala IS, Pinto AT, Ministro A, Poli E, Diegues IM, Pina F, Benadjaoud MA, Flamant S, Tamarat R, Os, ó, rio H, Pais D, Casal D, Pinto FJ, Matthiesen R, Fiuza M, Constantino Rosa Santos S, Molecular Changes In Cardiac Tissue As A New Marker To Predict Cardiac Dysfunction Induced By Radiotherapy. Front Oncol, 12():945521(2022) [pubmed]

submitter keyword: Cardiooncology, Radiotherapy, GLS, Cardiac dysfunction, Cardiac tissue, Cardiac muscle

Susana Constantino Rosa Santos
contact affiliation	Angiogenesis Laboratory, Centro Cardiovascular da Universidade de Lisboa (CCUL@RISE), Lisbon School of Medicine of the Universidade de Lisboa; Lisbon, 1649-028, Portugal
contact email	sconstantino@medicina.ulisboa.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033194
     1. Label: PRIDE project
     2. Name: Cardiac tissue remodeling as a new marker to predict cardiac dysfunction induced by radiotherapy.