PXD033191

PXD033191 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics analysis of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin
Description	The present study is a pioneering investigation that has been carefully designed to study proteomic effects of specific cancer-related mutation using the lgl mutant of D. melanogaster that has not been well characterised thus far. Recent findings concerning the D. melanogaster cancer models suggest that this model is well suited for study of cancer mechanisms and therapeutic interventions, especially in the brain and intestines (Mirzoyan et al., 2019). In addition, the integration of circadian biology into cancer research offers new insights into the implications of optimal timing for cancer treatment that could potentially reduce undesirable side effects and enhance prognosis (Ballesta et al., 2017). As a result, label free quantitative mass spectrometry was employed to investigate tissue-specific protein expression in head and intestinal tissues of D. melanogaster lgl mutants which was collected at 6-hours intervals throughout the 24-hour period. Furthermore, the efficacy of melatonin as an anticancer agent at the proteome level was also investigated. Together, the resulting information could potentially identify malfunctioning signalling pathways and circadian rhythm-dependent protein markers during tumorigenesis, as well as the mechanism of actions of melatonin on these processes, post-treatment
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:02.343.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaime Jayapalan
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-13 02:30:43	ID requested
1	2023-05-10 09:34:47	announced
⏵ 2	2023-11-14 08:46:08	announced	2023-11-14: Updated project metadata.

Wong KC, Jayapalan JJ, Subramanian P, Ismail MN, Abdul-Rahman PS, Label-free quantitative mass spectrometry analysis of the circadian proteome of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin. Arch Insect Biochem Physiol, 113(2):e22008(2023) [pubmed]

submitter keyword: label-free quantitative proteomics, lethal giant larvae,Circadian proteome, melatonin., Drosophila melanogaster

Jaime Jacqueline Jayapalan
contact affiliation	Department of Molecular Medicine, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia
contact email	jaime_jacklyn@um.edu.my
lab head
Jaime Jayapalan
contact affiliation	University of Malaya
contact email	jaime_jacklyn@um.edu.my
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033191

PRIDE project URI

• PRIDE
  1. PXD033191
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics analysis of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin