PXD033188

PXD033188 is an original dataset announced via ProteomeXchange.

Title	Extracellular Matrix Microenviroment-induced Bladder Smooth Muscle Proliferation in Bladder Fibrosis Progression
Description	We assessed the pivotal effect of YAP on the proliferation of human bladder smooth muscle cells (hBdSMCs) under different ECM stiffnesses. We investigated the interaction between YAP and Smad3 through mass spectrometry and immunoprecipitation (IP). Transcriptome sequencing was used to predict the phenotype and signalling pathways. CUT and TAG sequencing was utilized to analyse the target genes of Smad3, and a dual-luciferase reporter assay was used to confirm the sequencing results. The YAP inhibitor CA3 was used in a partial bladder outlet obstruction (pBOO) rat model construction to evaluate bladder smooth muscle proliferation in vivo.
HostingRepository	iProX
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-11-02_21:52:10.333.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xingpeng Di
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-12 23:55:03	ID requested
⏵ 1	2022-11-02 21:52:11	announced

Di XP, Jin X, Ai JZ, Xiang LY, Gao XS, Xiao KW, Li H, Luo DY, Wang KJ, YAP/Smad3 promotes pathological extracellular matrix microenviroment-induced bladder smooth muscle proliferation in bladder fibrosis progression. MedComm (2020), 3(4):e169(2022) [pubmed]

submitter keyword: Extracellular matrix stiffness, YAP, Smad3, Bladder fibrosis, Proliferation

Xi Jin
contact affiliation	Institute of Urology (Reconstructive Urology), West China Hospital, Sichuan University
contact email	10275681@qq.com
lab head
Xingpeng Di
contact affiliation	Sichuan University West China Hospital
contact email	1017609868@qq.com
dataset submitter

iProX dataset URI