PXD033185

PXD033185 is an original dataset announced via ProteomeXchange.

Title	TMT-Based Quantitative Proteomics Reveals Implication of a Late Embryogenesis Abundant Protein (BnLEA57) In Seed Oil Accumulation in Brassica napus L.
Description	Enhancing oil content is one of the major goals in Brassica napus breeding, however, genetic regulation of seed oil content in plants is complex and not fully elucidated. In the present study, we report proteins that were differentially accumulated in immature seeds of 35 days after anthesis between two recombinant inbred lines with contrasting seed oil content, HOCL (High oil content line) and LOCL (Low oil content line) using a multiplex isobaric tandem mass tags (TMT)-based quantitative proteomics approach. Over 4600 proteins were quantified in seeds of the two lines, and 342 proteins showed differential accumulation between seeds of HOCL and LOCL. Gene Ontology enrichment analysis revealed that the differentially-accumulated proteins were enriched in proteins involved in lipid biosynthesis and metabolism, photosynthesis, and nutrient reservoir activity. Western blot confirmed the increased abundance of a Late Embryogenesis Abundant protein (BnLEA57) in HOCL seeds compared with LOCL seeds, and over-expression of either BnLEA57 gene or its homology BnLEA55in transgenic Arabidopsis thaliana enhancedoil content in Arabidopsis seeds. Our work provides new insights into the molecular regulatory mechanism of seed oil content in B. napus.
HostingRepository	iProX
AnnounceDate	2022-04-12
AnnouncementXML	Submission_2022-09-29_20:43:20.496.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiping Deng
SpeciesList	scientific name: Brassica napus; NCBI TaxID: 3708;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-12 19:35:47	ID requested
1	2022-04-12 19:36:07	announced
⏵ 2	2022-09-29 20:43:21	announced	2022-09-30: Update publication information.

Zhou Z, Lin B, Tan J, Hao P, Hua S, Deng Z, L. Front Plant Sci, 13():907244(2022) [pubmed]

submitter keyword: seeds, quantitative proteomics, oil content, rapeseed, Brassica napus,LEA protein,late embryogenesis abundant protein,TMT

Zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
lab head
Zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
dataset submitter

iProX dataset URI