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PXD033183

PXD033183 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCellular and Comparative Proteomic Analyses Reveal Developmental Differences in Anthers at Meiosis between Diploid and Autotetraploid Rice
DescriptionNewly synthetic autotetraploid rice generally shows lower pollen fertility and seed setting rate in comparison to diploid rice, and this can hinder its own domestication and breeding. In this study, cytological analysis was used to demonstrate the imbalanced separation of homologous chromosomes occurred at meiosis I during the pollen mother cell development, and the early degeneration of tapetum cells in autotetraploid rice. We identified a total of 941 differentially expressed proteins (DEPs) in anthers, including 489 up-regulated and 452 down-regulated proteins. KOG analysis showed that the identified DEPs were related to post-translational modifications, such as protein ubiquitination. These modifications are related to chromatin remodeling and homologous recombination abnormalities during meiosis. The key proteins related to the pentose phosphate pathway (BGIOSGA016558, BGIOSGA022166, BGIOSGA028743) are significantly down-regulated, which can affect the failure of autotetraploid rice to provide the energy needed for cell development after polyploidization, and ultimately lead to the early degradation of the tapetum. Proteins (BGIOSGA017346and BGIOSGA027368) related to the degradation of glutenin are up-regulated, indicating that the decomposition of glutenin can lead to the early degradation of tapetum. These cytological and molecular evidences will help to promote our understanding of the differences in anther development between diploid and autotetraploid rice during meiosis.
HostingRepositoryiProX
AnnounceDate2022-04-12
AnnouncementXMLSubmission_2022-11-02_21:51:47.697.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTianya Ku
SpeciesList scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-12 19:31:15ID requested
12022-11-02 21:51:49announced
Publication List
Ku T, Gu H, Li Z, Tian B, Xie Z, Shi G, Chen W, Wei F, Cao G, Developmental Differences between Anthers of Diploid and Autotetraploid Rice at Meiosis. Plants (Basel), 11(13):(2022) [pubmed]
Keyword List
submitter keyword: Polyploidy, Rice, Autotetraploid, Meiosis, fertility, proteomics
Contact List
Gangqiang Cao
contact affiliationCollege of Agriculture, Zhengzhou University
contact emailcaogq@zzu.edu.cn
lab head
Tianya Ku
contact affiliationCollege of Agriculture, Zhengzhou University
contact emailkxm0305@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI