PXD033170 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Immunogenicity of mycobacterial extracellular vesicles isolated from host-related conditions informs about tuberculosis disease status |
| Description | Tuberculosis (TB) still represents a major global health problem affecting over 10 million people worldwide. At present, there is an urgent need for defining prognostic parameters, differential diagnostic, and correlates of protection from the disease. The gold standard test for TB diagnosis is still smear microscopy, but insufficiently detects pulmonary disease, besides the necessity of having laboratory infrastructures and be time-consuming. Both LAM urine test and Xpert MTB/RIF have been major game changers in this context. However, the low sensitivity of the former and the considerable delay of sample delivery of the latter, make the improvement of the TB diagnostic landscape a priority. The development of rapid point-of-care (POC)-based testing approaches would represent an important breakthrough in TB diagnostics in view of these short-comings. Most forms of life produce extracellular vesicles (EVs) and since the first detection of bacterial EVs more than 60 years, subsequent studies have demonstrated their functional commonality despite differences in bacterial cell envelope architecture. We demonstrated that Mycobacterium tuberculosis (Mtb), the causative agent of TB, produces EVs in vitro and in vivo as part of a sophisticated mechanism to manipulate host cellular physiology and to evade the host immune system. In a previous serology study, Mtb EVs (MEVs) were used to investigate their potential role as biomarkers to discern between different forms of disease status. It was shown that the recognition of several MEV associated proteins could have diagnostic properties. In this study, we pursued to expand the capabilities of MEVs in the context of TB diagnostics by analyzing the composition of MEVs isolated from Mtb cultures submitted to iron starvation and, testing their immunogenicity against a new cohort of serum samples including TB+, LTBI and healthy donors. We found that MEVs differ in protein composition when Mtb is under host-related stress yet MEVs seem to carry antigens that could serve as bonafide markers for direct detection. In addition, TB serology revealed three new MEV antigens with great biomarker capacity. These results encourage investigating the feasibility of the development of a POC device based on selected MEV-associated proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:38:30.830.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD033170 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mikel Azkargorta |
| SpeciesList | scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-04-12 02:35:00 | ID requested | |
| 1 | 2022-08-02 08:23:41 | announced | |
| 2 | 2023-11-14 08:56:20 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 3 | 2024-10-22 05:38:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: serology.,Mycobacterium tuberculosis, extracellular vesicles, immunogenicity, diagnostic biomarkers |
Contact List
| Felix Elortza |
|---|
| contact affiliation | Proteomics Service CIC bioGUNE Build. 800 Bizkaia Tech. Park 48160 Derio (Spain) |
| contact email | felortza@cicbiogune.es |
| lab head | |
| Mikel Azkargorta |
|---|
| contact affiliation | Proteomics Platform CIC bioGUNE |
| contact email | mazkargorta@cicbiogune.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033170
- Label: PRIDE project
- Name: Immunogenicity of mycobacterial extracellular vesicles isolated from host-related conditions informs about tuberculosis disease status
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