PXD033169 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Novel Diagnostic Biomarkers for High-Grade Serous Ovarian Cancer Uncovered by Data-Independent Acquisition Mass Spectrometry |
| Description | High-grade serous ovarian cancer (HGSOC) represents the major histological type of ovarian cancer (OC), and lack of effective screening tools and early detection methods significantly contributes to the poor prognosis of HGSOC. Currently, there is no reliable diagnostic biomarkers for HGSOC. In this study, we performed liquid chromatography data-independent acquisition tandem-mass spectrometry on depleted serum samples from 26 HGSOC cases and 24 healthy controls (HCs) to discover potential HGSOC diagnostic biomarkers. A total of 1,847 proteins were identified across all samples, among which 116 proteins showed differential expressions between HGSOC patients and HCs. Network modeling showed activations of coagulation and complement cascades, platelet activation and aggregation, NET formation, TLR4, IGF, and TGF-β signaling, and suppression of lipoprotein assembly and FcγR activation in HGSOC. Based on the network model, we prioritized 28 biomarker candidates, and validated 18 of them using targeted MS assays in an independent cohort. Predictive modeling showed a sensitivity of 1 and specificity of 0.91 in the validation cohort. Finally, in vitro functional assays on four potential biomarkers (FGA, VWF, ARHGDIB, and SERPINF2) suggested that they may play an important role in cancer cell proliferation and migration in HGSOC. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_08:56:05.666.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sunghyun Huh |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-04-12 02:34:35 | ID requested | |
| 1 | 2022-08-11 21:38:53 | announced | |
| ⏵ 2 | 2023-11-14 08:56:06 | announced | 2023-11-14: Updated project metadata. |
Publication List
| Huh S, Kang C, Park JE, Nam D, Kim SI, Seol A, Choi K, Hwang D, Yu MH, Chung HH, Lee SW, Kang UB, Novel Diagnostic Biomarkers for High-Grade Serous Ovarian Cancer Uncovered by Data-Independent Acquisition Mass Spectrometry. J Proteome Res, 21(9):2146-2159(2022) [pubmed] |
Keyword List
| submitter keyword: proteomics, mass spectrometry, data-independent acquisition,serum biomarker, high-grade serous ovarian cancer |
Contact List
| Un-Beom Kang |
|---|
| contact affiliation | Bertis R&D Division, Bertis Inc., Republic of Korea |
| contact email | unbeom.kang@bertis.com |
| lab head | |
| Sunghyun Huh |
|---|
| contact affiliation | Bertis |
| contact email | sunghyun.huh@bertis.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033169 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033169
- Label: PRIDE project
- Name: Novel Diagnostic Biomarkers for High-Grade Serous Ovarian Cancer Uncovered by Data-Independent Acquisition Mass Spectrometry
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