PXD033162

PXD033162 is an original dataset announced via ProteomeXchange.

Title	Differences in the proteomic and metabolomic response during the early stages of Phytophthora cinnamomi infection of two Quercus species with contrasting degrees of susceptibility.
Description	Phytophthora cinnamomi Rands is a cosmopolite and phyllophagous pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. Despite Chinese cork tree (Quercus variabilis Blume) forests native to Asia being generally healthy, the populations of cork trees (Quercus suber L.) in Europe have been decimated by P. cinnamomi. The present study tries to identify the differences in the early proteomic and metabolomic response of these two tree species that lead to their contrasting susceptibility to P. cinnamomi attack. By using micropropagated clonal plants, we tried to minimize the plant-to-plant differences in the defense response that is maximized by the high intraspecific genetic variability inherent to the Quercus genus. The evolution on the content of Phytophthora proteins in the roots during the first 36 hours after inoculation suggest a slower infection process in Q. variabilis plants. These plants displayed a significant decrease in sugars in the roots, together with a downregulation of proteins related to carbon metabolism. In the leaves, the biggest changes in proteomic profiling were observed 16 hours after inoculation. and included increased abundance of peroxidases, superoxidedismutases and gluthatione S-transferases in Q. variabilis plants, which probably aided its resistance against P. cinnamomi attack.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:22.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Quercus suber (Cork oak); NCBI TaxID: 58331; scientific name: Quercus variabilis; NCBI TaxID: 103481;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-12 01:55:37	ID requested
1	2022-08-02 10:42:23	announced
⏵ 2	2023-11-14 08:56:22	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Gluthatione S-transferase, phenylpropanoids, Resistance, Sugars, Peroxidases,Micropropagation, Plant Pathogen

Martin Cerny
contact affiliation	Department of Molecular Biology and Radiobiology, Mendel University in Brno, CZ
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033162
     1. Label: PRIDE project
     2. Name: Differences in the proteomic and metabolomic response during the early stages of Phytophthora cinnamomi infection of two Quercus species with contrasting degrees of susceptibility.