PXD033132

PXD033132 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of LMNA-deficiency microglia activation
Description	To study the role of LMNA gene in the central nervous system, LMNA gene knock-out mice model and BV2 cell line were obtained. Microglia activation was detected in mice and BV2 cell lines. Phagocytic activity and cytokine production were determined with immunofluorescent and qPCR respectively. Protein profiling of primary microglia and BV2 cells was identified with LC-MS/MS. Differentially expressed proteins were validated by western blotting.
HostingRepository	iProX
AnnounceDate	2022-04-08
AnnouncementXML	Submission_2023-01-03_02:02:44.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haotian_Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-10 19:34:58	ID requested
⏵ 1	2023-01-03 02:02:45	announced

Dataset with its publication pending

submitter keyword: TMT, primary microglia, LMNA-knockout mouse, LMNA-knockdown

Wei_Ge
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Haotian_Liu
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact email	liuhaotian@ibms.pumc.edu.cn
dataset submitter

iProX dataset URI