PXD033131

PXD033131 is an original dataset announced via ProteomeXchange.

Title	Hyaluronan And Proteoglycan Link Protein 1 (HAPLN1) promotes Proliferation and Pro-inflammatory phenotype but not mobility of Fibroblast-like Synoviocytes
Description	Label-free proteomics study was applied to FLSs transfected by si-HAPLN1, or treated by rHAPLN1 (50 ng/ml) and their controls for 48 h by PTMBiolabs, Inc. (Hangzhou, China). Each concentration was tested with 3 biological replicates. LC−MS/MS proteomics analysis was performed on an EASY-nLC 1000 ultra-performance liquid chromatography (UPLC) system, followed by MS/MS using Q Exactive Plus (ThermoFisher Scientific, USA) coupled online to the UPLC system. The MS/MS data were retrieved by the Maxquant search engine (v1.6.6.0). A human database was searched (Swiss-Prot). The decoy database anti-library was used to reduce the false positive rate (FDR). The FDR was adjusted to < 1%, and the minimum score for modified peptides was set > 40. Proteins with a fold-change ≥1.50 or ≤0.67 between si-HAPLN1, rHAPLN1 and their controls were considered as expression significant. Based on the protein sequence alignment method, the protein domain functions were defined by InterProScan (http://www.ebi.ac.uk/interpro/).
HostingRepository	iProX
AnnounceDate	2022-04-08
AnnouncementXML	Submission_2023-04-03_00:39:09.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-10 19:29:50	ID requested
1	2023-04-02 02:43:45	announced
⏵ 2	2023-04-03 00:39:10	announced	2023-04-03: Update publication information.

Chen Y, Wang B, Chen Y, Wu Q, Lai WF, Wei L, Nandakumar KS, Liu D, HAPLN1 Affects Cell Viability and Promotes the Pro-Inflammatory Phenotype of Fibroblast-Like Synoviocytes. Front Immunol, 13():888612(2022) [pubmed]

submitter keyword: Rheumatoid arthritis, hyaluronan and proteoglycan link protein 1, fibroblast-like synoviocytes, cell viability, cell migration, pathogenesis

Dong Zhou
contact affiliation	Shenzhen People's Hospital
contact email	liu_dz2001@sina.com
lab head
Yong Chen
contact affiliation	Shenzhen People's Hospital
contact email	chenyong_2022@126.com
dataset submitter

iProX dataset URI