PXD033127

PXD033127 is an original dataset announced via ProteomeXchange.

Title	Human tau 0N4R isoform hyperphosphorylation LC-MS/MS
Description	Soluble aggregates of the microtubule-associated protein tau have been challenging to assemble and characterize, despite their important role in the development of tauopathies. We found that sequential hyperphosphorylation by protein kinase A in conjugation with either glycogen synthase kinase 3betaor stress activated protein kinase 4 enabled recombinant wild-type tau of isoform 0N4R to spontaneously polymerize into small amorphous aggregates in vitro. We employed tandem mass spectrometry to determine the phosphorylation sites.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_07:47:31.054.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Meng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 13:51:44	ID requested
⏵ 1	2022-04-13 07:47:31	announced

Dataset with its publication pending

submitter keyword: hyperphosphorylation, tau,human, LC-MS/MS

David Klenerman
contact affiliation	University of Cambridge, Department of Chemistry
contact email	dk10012@cam.ac.uk
lab head
Jonathan Meng
contact affiliation	University of Cambridge
contact email	jxm21@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD033127

PRIDE project URI

• PRIDE
  1. PXD033127
     1. Label: PRIDE project
     2. Name: Human tau 0N4R isoform hyperphosphorylation LC-MS/MS