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PXD033125

PXD033125 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGRAMD1C regulates autophagy initiation and mitochondrial bioenergetics
DescriptionDuring autophagy, cytosolic cargo is sequestered into double-membrane vesicles called autophagosomes. The origin and identity of the membranes that form the autophagosome remain to be fully characterized. Here, we investigated the role of cholesterol in starvation-induced autophagy and identify a role for the ER-localized cholesterol transport protein GRAMD1C in the regulation of autophagy and mitochondrial function. We demonstrate that cholesterol depletion leads to a rapid induction of autophagy and a corresponding increased abundance of curved autophagy membranes. We further show that GRAMD1C is a negative regulator of starvation-induced autophagy. Similar to its yeast orthologue, GRAMD1C is recruited to mitochondria through its GRAM domain. Additionally, we find that GRAMD1C depletion leads to increased mitochondrial cholesterol accumulation and mitochondrial oxidative phosphorylation. Finally, we demonstrate that expression of GRAM family genes is linked to clear cell renal carcinoma survival, highlighting the pathophysiological relevance of cholesterol transport proteins.
HostingRepositoryPRIDE
AnnounceDate2022-10-19
AnnouncementXMLSubmission_2022-10-18_22:23:51.177.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuulaNyman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-08 13:50:58ID requested
12022-10-18 22:23:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: GRAMD1C, MBCD, Autophagy, GRAM, Cholesterol
Contact List
TuulaNyman
contact affiliationHead of Proteomics
contact emailtuula.nyman@medisin.uio.no
lab head
TuulaNyman
contact affiliationUniversity of Oslo
contact emailtuula.nyman@medisin.uio.no
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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