PXD033122 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ribosome impairment regulates intestinal stem cell identity via ZAKa activation. |
Description | The small intestine is a rapidly proliferating organ that is maintained by a small population of Lgr5-expressing intestinal stem cells (ISCs). However, several Lgr5-negative ISC populations have been identified, and this remarkable plasticity allows the intestine to rapidly respond to both the local environment and to damage. The mediators of such plasticity are still largely unknown. Using intestinal organoids and mouse models, we show that upon ribosome impairment (driven by Rptor deletion, amino acid starvation, or low dose cyclohexamide treatment) ISCs gain an Lgr5-negative, fetal-like identity. This is accompanied by a rewiring of metabolism. Our findings suggest that the ribosome can act as a sensor of nutrient availability, allowing ISCs to respond to the local nutrient environment. Mechanistically, we show that this phenotype requires the activation of ZAKÉ‘, which in turn activates YAP, via SRC. Together, our data reveals a central role for ribosome dynamics in intestinal stem cells, and identify the activation of ZAKÉ‘ as a critical mediator of stem cell identity. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-13 |
AnnouncementXML | Submission_2022-06-13_03:37:33.632.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liesbeth Hoekman |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-08 13:39:23 | ID requested | |
⏵ 1 | 2022-06-13 03:37:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS, proteomics, ZAKa, intestinal stem cells, |
Contact List
Maarten Altelaar |
contact affiliation | Proteomics Facility, Netherlands Cancer Institute, Amsterdam, Netherlands; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Centre, Utrecht, The Netherlands. |
contact email | m.altelaar@nki.nl |
lab head | |
Liesbeth Hoekman |
contact affiliation | The Netherlands Cancer Institute, Amsterdam, The Netherlands. |
contact email | l.hoekman@nki.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD033122
- Label: PRIDE project
- Name: Ribosome impairment regulates intestinal stem cell identity via ZAKa activation.