PXD033111

PXD033111 is an original dataset announced via ProteomeXchange.

Title	An organotypic model of human skin simulates the dermonecrotic effects of Naja nigricollis envenoming
Description	Snakebite envenoming is a neglected tropical disease that kills and maims hundreds of thousands annually. Naja nigricollis, the black-necked spitting cobra, has a cytotoxin-rich venom that is able to cause severe dermonecrosis and is not efficiently neutralized by current antivenoms. Here, we introduce an organotypic model of human skin to study the effects of exposure to N. nigricollis venom on human cells and compare it to the currently available in vivo mouse model. Histologically, the organotypic model simulates the severe necrotic lesions observed in mice. Proteomically, we show that among widespread global changes in protein abundance, many pathways involved in skin homeostasis and wound healing are specifically affected in both models. These results are the first to suggest that this organotypic model can simulate dermonecrosis caused by N. nigricollis venom and could thus be used to bridge the gap between in vitro and animal-based experiments for the study of the venom-induced cytotoxicity. This is an initial step towards replacing such animal-based experiments, which are associated with pain and tissue damage. The organotypic model may also find utility in evaluating the efficacy of new therapeutics against the severe and long-lasting consequences of snakebite envenoming in humans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:13.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich auf dem Keller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 09:49:20	ID requested
1	2023-01-11 08:59:45	announced
⏵ 2	2023-11-14 08:25:15	announced	2023-11-14: Updated project metadata.

Ahmadi S, Pachis ST, Kalogeropoulos K, McGeoghan F, Canbay V, Hall SR, Crittenden EP, Dawson CA, Bartlett KE, Guti, é, rrez JM, Casewell NR, Keller UAD, Laustsen AH, Proteomics and histological assessment of an organotypic model of human skin following exposure to Naja nigricollis venom. Toxicon, 220():106955(2022) [pubmed]

submitter keyword: Dermonecrosis, Snakebite envenoming, Naja nigricollis, Skin organotypic,Proteomics, Cytotoxin, Keratinocyte, 3D cell culture, DDA, LC-MS/MS

Ulrich auf dem Keller
contact affiliation	DTU Bioengineering
contact email	uadk@dtu.dk
lab head
Ulrich auf dem Keller
contact affiliation	Technical University of Denmark
contact email	ulrich.aufdemkeller@biol.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033111
     1. Label: PRIDE project
     2. Name: An organotypic model of human skin simulates the dermonecrotic effects of Naja nigricollis envenoming