PXD033108
PXD033108 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer |
Description | We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS, n=30) and validation (Australian Ovarian Cancer Study, n=95) cohorts using shotgun and targeted proteomics, respectively. A 7-lectin discovery screen shortlisted 60 candidate proteins and 3 lectins for validation, which revealed elevated levels of AAL, SNA or STL-binding FIBB, CO9, ITIH3, HPT, A1AT, AACT in HGSOC, while IBP3, A2MG, PON1, CHLE and ALS were reduced. Multimarker panels were developed using generalized regression with lasso estimation and leave-one-out cross-validation. The best performing panel comprised of 13 peptides from Solanum Tuberosum lectin (STL)-binding proteins with 96.3% area under the receiver operating curve, 97.7% specificity and 78.6% sensitivity for distinguishing HGSOC from benign and healthy groups. The peptides robust in cross-validations were from IBP3, KNG1, CO9, THRB, HPTR, HPT, FINC, FA10, GELS. The validated serum biomarkers show promise for early detection of HGSOC and should be further evaluated. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2024-08-06 |
AnnouncementXML | Submission_2024-08-06_14:23:54.105.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | supported by repository but incomplete data and/or metadata |
PrimarySubmitter | Mriga Dutt |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(5)15N(1) |
Instrument | 6490 Triple Quadrupole LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-04-08 09:31:12 | ID requested | |
⏵ 1 | 2024-08-06 14:23:54 | announced |
Publication List
Dutt M, Hartel G, Richards RS, Shah AK, Mohamed A, Apostolidou S, Gentry-Maharaj A, Hooper JD, Perrin LC, Menon U, Hill MM, Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer. Proteomics Clin Appl, 17(4):e2200114(2023) [pubmed] |
Keyword List
submitter keyword: glycosylation, LeMBA, lectin, ovarian cancer screening, STL |
Contact List
Michelle Hill | |
---|---|
contact affiliation | QIMR Berghofer Medical Research Institute |
contact email | michelle.hill@qimrberghofer.edu.au |
lab head | |
Mriga Dutt | |
contact affiliation | QIMR Berghofer Medical Research Institute |
contact email | dutt.mriga@gmail.com |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |