PXD033105

PXD033105 is an original dataset announced via ProteomeXchange.

Title	Soluble guanylyl cyclase mediates noncanonical nitric oxide signaling by nitrosothiol transfer under oxidative stress
Description	Soluble guanylyl cyclase (GC1) is an α/β heterodimer producing cGMP when stimulated by nitric oxide (NO). The NO-GC1-cGMP pathway is essential to cardiovascular homeostasis but is disrupted by oxidative stress, which induces GC1 desensitization to NO by S-nitrosation (SNO) of its cysteines (C). We discovered that under these conditions, GC1-α subunit increases cellular S-nitrosation via transfer of its nitrosothiols to other proteins (transnitrosation). One of the SNO-targets was the oxidized form of the oxido-reductase Thioredoxin1 (oTrx1), which is unilaterally transnitrosated by GC1. GC1-αC610 was a major SNO-donor to oTrx1-C73. Because oTrx1 itself drives transnitrosation, we sought and identified several SNO-proteins targeted by both GC1 and oTrx1. Among them, transnitrosation of RhoA by SNO-GC1 requires oTrx1 as a nitrosothiol relay, suggesting a SNO-GC1→oTrx1→RhoA cascade. We showed that RhoA pathway, which is antagonized by the canonical NO-cGMP signaling, was alternatively inhibited by GC1-α-dependent S-nitrosation under oxidative conditions. We propose that some SNO-GC1’ functions are adaptive responses triggered by oxidation of the canonical NO-cGMP pathway
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:32:07.240.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tong Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 03:09:36	ID requested
1	2022-10-14 20:49:40	announced
⏵ 2	2023-11-14 08:32:09	announced	2023-11-14: Updated project metadata.

10.6019/PXD033105;

Cui C, Wu C, Shu P, Liu T, Li H, Beuve A, Soluble guanylyl cyclase mediates noncanonical nitric oxide signaling by nitrosothiol transfer under oxidative stress. Redox Biol, 55():102425(2022) [pubmed]

submitter keyword: nitric oxide, S-nitrosylation, cGMP, S-nitrosation, oxidative stress, soluble guanylyl cyclase

Annie Beuve
contact affiliation	Department Pharmacology, Physiology and Neurosciences NJMS- RUTGERS
contact email	beuveav@njms.rutgers.edu
lab head
Tong Liu
contact affiliation	Rutgers University
contact email	linto@njms.rutgers.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033105
     1. Label: PRIDE project
     2. Name: Soluble guanylyl cyclase mediates noncanonical nitric oxide signaling by nitrosothiol transfer under oxidative stress