PXD033100

PXD033100 is an original dataset announced via ProteomeXchange.

Title	Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop
Description	Rearrangement hot spots (Rhs) proteins are members of the broad family of polymorphic toxins. Polymorphic toxins are modular proteins composed of an N-terminal region that specifies their mode of secretion into the medium or into the target cell, a central delivery module and a C-terminal domain that has toxic activity. Here, we structurally and functionally characterize the C-terminal toxic domain of the antibacterial Rhsmain protein, which is delivered by the Type VI secretion system of Salmonella enterica Typhimurium. We show that this domain adopts an ADP-ribosyltransferase fold and inhibits protein synthesis by transferring an ADP-ribose group from NAD+ to the elongation factor EFTu. This modification is specifically placed on the sidechain of the conserved D21 residue located on the P-loop of the EF-Tu G-domain. Finally, we demonstrate that its cognate immunity protein neutralizes Rhsmain C-terminal toxin activity by acting like a lid that closes the catalytic site and traps the NAD+.
HostingRepository	PRIDE
AnnounceDate	2023-03-11
AnnouncementXML	Submission_2023-03-11_03:48:10.076.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MartialRey
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 01:25:35	ID requested
⏵ 1	2023-03-11 03:48:10	announced

Jur, ė, nas D, Rey M, Byrne D, Chamot-Rooke J, Terradot L, Cascales E, Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop. Nucleic Acids Res, 50(22):13114-13127(2022) [pubmed]

submitter keyword: elongation factor, ADPribosylation, toxin, rearrangement hot spots,T6SS, P-loop, modification, translation

JuliaChamot-Rooke
contact affiliation	Mass Spectrometry for Biology Unit, CNRS USR2000, Institut Pasteur, CNRS, 28 rue du Dr Roux, Paris 75015, France.
contact email	julia.chamot-rooke@pasteur.fr
lab head
MartialRey
contact affiliation	CNRS, Pasteur
contact email	martial.rey@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033100

PRIDE project URI

• PRIDE
  1. PXD033100
     1. Label: PRIDE project
     2. Name: Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop