PXD033092

PXD033092 is an original dataset announced via ProteomeXchange.

Title	Title（Project name）Proteomic Investigation of the Molecular Mechanisms in Response to the PEG-Induced Drought Stress in Soybean Roots
Description	Roots are generally the critical drought sensors, but little is known about their molecular response to drought stress. We used the drought-tolerant soybean variety ‘Jiyu 47’ to investigate the differentially expressed proteins (DEPs) in soybean roots during the seedling stage based on the TMT proteomics analysis. Results of enrichment analyses based on a total of 468 DEPs revealed a coordinated expression pattern of proteins involved in various cellular metabolisms responding to drought stress in soybean roots. Our results showed that drought stress caused significant alterations in the expression of proteins involved in several metabolic pathways in soybean roots, including the carbohydrate metabolism, the metabolism of the osmotic regulation substances, and the antioxidant defense system (i.e., the glutathione metabolism). Increased production of reduced glutathione (GSH) enhanced the prevention of the damage caused by reactive oxygen species and the tolerance of the abiotic stress. The glutathione metabolism played a key role in modifying the antioxidant defense system in response to drought stress in soybean roots. Our proteomic study demonstrated that the soybean plants responded to drought stress by coordinating their protein expression during the vegetative stage, providing novel insights into the molecular mechanisms regulating the response to abiotic stress in plants.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:06.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Zhou
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 08:37:04	ID requested
1	2022-05-08 03:59:47	announced
⏵ 2	2023-11-14 08:30:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: soybean root

drought stress

physiological response

proteomics

antioxidant

enrichment analysis

Ying Zhou
contact affiliation	College of Life Sciences, Jilin Agricultural University, Changchun 130118,China
contact email	yzhou@jlau.edu.cn
lab head
Ying Zhou
contact affiliation	College of Life Sciences, Jilin Agricultural University, Changchun
contact email	yzhou@jlau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD033092

PRIDE project URI

• PRIDE
  1. PXD033092
     1. Label: PRIDE project
     2. Name: Title（Project name）Proteomic Investigation of the Molecular Mechanisms in Response to the PEG-Induced Drought Stress in Soybean Roots