PXD033091

PXD033091 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human neuronal SH-SY5Y in neurological diseases exposed to phtalates and metaks
Description	This study aimed to reveal biological pathways involved in neurodevelopmental disorders after co-exposure to phthalates and metals by using a multi-omics analysis in a human neuronal cell line, SH-SY5Y. Mixtures of phthalates (DEHP, DiNP, BBzP) and metals (lead, methylmercury, total mercury, cadmium) were selected as they were the most abundant ones in the REPRO_PL and PHIME cohorts, respectively, in the frame of the EU project HEALS, investigating if exposure to those pollutants could be associated to neurodevelopmental disorders in children across Europe. Their concentrations in the human brain were calculated at the INTEGRA computational platform. The differentiated cell line was exposed to this mixture and a ten-times higher one for 4 days. Transcriptomic and proteomic analyses were performed and the data analyzed using advanced bioinformatics tools. Co-exposure to phthalates and metals leads to the perturbation of various metabolic pathways associated with neuronal diseases. The proteomic data showed that a number of neurological diseases are statistically associated with the exposure of the SH-SY5Y cells to the mixtures of pollutants. In conclusion, this study reveals that co-exposure to phthalates and metals disturbs biochemical processes in the brain model, which could be associated with clinical neurodevelopmental perturbations in cohorts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:18.106.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 08:34:33	ID requested
1	2023-12-20 10:09:39	announced
⏵ 2	2024-10-22 06:18:19	announced	2024-10-22: Updated project metadata.

10.1289/ehp7102;

Koual M, Tomkiewicz C, Guerrera IC, Sherr D, Barouki R, Coumoul X, Zebrafish Study. Environ Health Perspect, 129(3):37002(2021) [pubmed]

submitter keyword: neuron, high pH fractionation, brain,exposome, pathway analysis

Chiara Guerrera
contact affiliation	Proteomics Platform, SFR Necker INSERM US24, Université Paris Cité, 160 rue de Vaugirard, 75015 Paris, France
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD033091

PRIDE project URI

• PRIDE
  1. PXD033091
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human neuronal SH-SY5Y in neurological diseases exposed to phtalates and metaks