PXD033089 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome of Tunneling Nanotubes (TNTs) in U2OS cells; comparison with Extracellular Vesicles (EVs) |
Description | Tunneling nanotubes (TNTs) are thin F-actin based membranous structures that form continuous cytoplasmic bridges between cells over distances ranging from several hundred nm up to 100 µm. They allow cell-to-cell communication by facilitating the transfer of different cargoes directly from cytoplasma to cytoplasma of the connected cells, including organelles (lysosomes, mitochondria), micro or mRNAs, pathogens, and misfolded proteins (for example prion proteins, tau or α-synuclein aggregates). TNTs could play major roles in various diseases, including neurodegenerative diseases or cancers of different types. TNT formation is highly dynamic and depends on cellular stresses and actin regulators. However, the mechanisms of TNT formation and TNT molecular components and regulators are still poorly understood. Because of their size, of some common components, of the ability to transfer cellular material to remote cells and because TNTs are fragile and easily broken and released in cell culture supernatants (where EVs are also found), TNTs were difficult to distinguish from EVs for their composition and function. With the goal of identifying structural components of TNTs, beside actin filaments, and possibly markers and regulators of these structures, we established a protocol of purification from U2OS cultured cells, allowing to separate TNTs from extracellular vesicles (EVs) and from cell bodies. We obtained the full composition of TNTs, compared to EVs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-13 |
AnnouncementXML | Submission_2024-09-13_09:39:47.598.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MARIETTE MATONDO |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-07 07:28:23 | ID requested | |
⏵ 1 | 2024-09-13 09:39:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS,TNTs, Exosomes, Extracellular vesicles |
Contact List
Christel Brou |
contact affiliation | Trafic membranaire et Pathogénèse, Institut Pasteur, Paris |
contact email | christel.brou@pasteur.fr |
lab head | |
MARIETTE MATONDO |
contact affiliation | Pasteur |
contact email | mariette.matondo@pasteur.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033089
- Label: PRIDE project
- Name: The proteome of Tunneling Nanotubes (TNTs) in U2OS cells; comparison with Extracellular Vesicles (EVs)