PXD033089

PXD033089 is an original dataset announced via ProteomeXchange.

Title	The proteome of Tunneling Nanotubes (TNTs) in U2OS cells; comparison with Extracellular Vesicles (EVs)
Description	Tunneling nanotubes (TNTs) are thin F-actin based membranous structures that form continuous cytoplasmic bridges between cells over distances ranging from several hundred nm up to 100 µm. They allow cell-to-cell communication by facilitating the transfer of different cargoes directly from cytoplasma to cytoplasma of the connected cells, including organelles (lysosomes, mitochondria), micro or mRNAs, pathogens, and misfolded proteins (for example prion proteins, tau or α-synuclein aggregates). TNTs could play major roles in various diseases, including neurodegenerative diseases or cancers of different types. TNT formation is highly dynamic and depends on cellular stresses and actin regulators. However, the mechanisms of TNT formation and TNT molecular components and regulators are still poorly understood. Because of their size, of some common components, of the ability to transfer cellular material to remote cells and because TNTs are fragile and easily broken and released in cell culture supernatants (where EVs are also found), TNTs were difficult to distinguish from EVs for their composition and function. With the goal of identifying structural components of TNTs, beside actin filaments, and possibly markers and regulators of these structures, we established a protocol of purification from U2OS cultured cells, allowing to separate TNTs from extracellular vesicles (EVs) and from cell bodies. We obtained the full composition of TNTs, compared to EVs.
HostingRepository	PRIDE
AnnounceDate	2024-09-13
AnnouncementXML	Submission_2024-09-13_09:39:47.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 07:28:23	ID requested
⏵ 1	2024-09-13 09:39:47	announced

Dataset with its publication pending

submitter keyword: LC-MSMS,TNTs, Exosomes, Extracellular vesicles

Christel Brou
contact affiliation	Trafic membranaire et Pathogénèse, Institut Pasteur, Paris
contact email	christel.brou@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD033089

PRIDE project URI

• PRIDE
  1. PXD033089
     1. Label: PRIDE project
     2. Name: The proteome of Tunneling Nanotubes (TNTs) in U2OS cells; comparison with Extracellular Vesicles (EVs)