PXD033086

PXD033086 is an original dataset announced via ProteomeXchange.

Title	PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM FASCIOLA HEPATICA HATCHING EGGS AND JUVENILES IN CULTURE
Description	The identification of extracellular vesicles (EVs) in Fasciola hepatica has provided a new way to understand parasite-host communication. Most of the studies on EVs have focused on the adult stage of F. hepatica, but recently, the presence of EVs from different developmental stages has been reported. To better understand the role of EVs in the infection process and immune response modulation, the protein cargo of EVs from embryonated eggs and newly-excysted juvenile (NEJs) flukes cultured up to 28 days, has been analyzed. EVs were isolated by size exclusion chromatography and evaluated by nanoparticle tracking analysis and transmission electron microscopy. LC-MS/MS proteomic analysis of EVs revealed the presence of 23 different proteins from embryonated egg-derived EVs and 29 different proteins from NEJ-derived EVs. Most of the identified proteins had been previously described in EVs from F. hepatica adults, including cytoskeletal proteins, glycolytic enzymes, stress-related proteins and tetraspanins. Nevertheless, EVs from hatching eggs and NEJs exhibited qualitative differences in composition, when compared to EVs form adults, including the absence of cathepsin cysteine peptidases. The differential content of the EVs released by the different developmental stages of the parasite reflect the intense activity of NEJs at this early stage, with several proteins involved in membrane traffic and cell physiology. This new set of identified proteins could help to understand key metabolic, biochemical and molecular mechanisms mediated by EVs that take place upon egg hatching and after parasite excystment.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:12:45.777.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Fasciola hepatica; NCBI TaxID: 6192;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 06:45:36	ID requested
⏵ 1	2022-08-12 00:12:46	announced

Trelis M, S, á, nchez-L, ó, pez CM, S, á, nchez-Palencia LF, Ram, í, rez-Toledo V, Marcilla A, Bernal D, Hatching Eggs and Juveniles in Culture. Front Cell Infect Microbiol, 12():903602(2022) [pubmed]

submitter keyword: Extracellular vesicles

Fasciola hepatica juveniles

Fasciola hepatica eggs

helminth proteomics

Dolores Bernal
contact affiliation	Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Burjassot (Valencia), Spain
contact email	M.Dolores.Bernal@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033086

PRIDE project URI

• PRIDE
  1. PXD033086
     1. Label: PRIDE project
     2. Name: PROTEOMIC ANALYSIS OF EXTRACELLULAR VESICLES FROM FASCIOLA HEPATICA HATCHING EGGS AND JUVENILES IN CULTURE