PXD033085
PXD033085 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphosites of the yeast centrosome component Spc110 contribute to mitotic exit |
Description | Spc110 is an essential component of the spindle pole body (SPB), the yeast equivalent of the centrosome, that recruits the gamma-tubulin complex to the nuclear side of SPB to form the mitotic spindle. Here we identify two phosphosites (S11 and S36) in old (from maternal origin) Spc110. Yeast expressing non-phosphorylatable Spc110S11AS36A were sensitive to the microtubule depolymerizing drug benomyl. In an undisturbed cell cycle, Spc110S11AS36A cells had normal spindles and cell cycle progression until late anaphase. However, Spc110S11AS36A cells were delayed in late-anaphase and presented elevated levels of Cdc14, a protein phosphatase required for mitotic exit, at the SPBs. Thus, we propose that the S11 and S36 phosphosites of Spc110 regulate Cdc14 localization at the SPBs for timely mitotic exit. |
HostingRepository | jPOST |
AnnounceDate | 2023-04-03 |
AnnouncementXML | Submission_2023-04-02_20:24:02.003.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Radoslaw Sobota |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; unknown modification; unknown modification |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-04-07 06:03:28 | ID requested | |
⏵ 1 | 2023-04-02 20:24:03 | announced |
Publication List
Abbasi M, Julner A, Lim YT, Zhao T, Sobota RM, Men, é, ndez-Benito V, Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit. Biol Open, 11(11):(2022) [pubmed] |
Keyword List
submitter keyword: microtubule |
mitosis |
spindle |
phosphorylation |
spindle pole body |
pericentrin, tubulin, Cdc14, MEN, yeast. |
Contact List
Victoria Menéndez-Benito | |
---|---|
lab head | |
Radoslaw Sobota | |
contact affiliation | IMCB A-STAR Singapore |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001557/ |