PXD033085

PXD033085 is an original dataset announced via ProteomeXchange.

Title	Phosphosites of the yeast centrosome component Spc110 contribute to mitotic exit
Description	Spc110 is an essential component of the spindle pole body (SPB), the yeast equivalent of the centrosome, that recruits the gamma-tubulin complex to the nuclear side of SPB to form the mitotic spindle. Here we identify two phosphosites (S11 and S36) in old (from maternal origin) Spc110. Yeast expressing non-phosphorylatable Spc110S11AS36A were sensitive to the microtubule depolymerizing drug benomyl. In an undisturbed cell cycle, Spc110S11AS36A cells had normal spindles and cell cycle progression until late anaphase. However, Spc110S11AS36A cells were delayed in late-anaphase and presented elevated levels of Cdc14, a protein phosphatase required for mitotic exit, at the SPBs. Thus, we propose that the S11 and S36 phosphosites of Spc110 regulate Cdc14 localization at the SPBs for timely mitotic exit.
HostingRepository	jPOST
AnnounceDate	2023-04-03
AnnouncementXML	Submission_2023-04-02_20:24:02.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; unknown modification; unknown modification
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 06:03:28	ID requested
⏵ 1	2023-04-02 20:24:03	announced

Abbasi M, Julner A, Lim YT, Zhao T, Sobota RM, Men, é, ndez-Benito V, Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit. Biol Open, 11(11):(2022) [pubmed]

submitter keyword: microtubule

mitosis

spindle

phosphorylation

spindle pole body

pericentrin, tubulin, Cdc14, MEN, yeast.

Victoria Menéndez-Benito
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001557/