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PXD033085

PXD033085 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphosites of the yeast centrosome component Spc110 contribute to mitotic exit
DescriptionSpc110 is an essential component of the spindle pole body (SPB), the yeast equivalent of the centrosome, that recruits the gamma-tubulin complex to the nuclear side of SPB to form the mitotic spindle. Here we identify two phosphosites (S11 and S36) in old (from maternal origin) Spc110. Yeast expressing non-phosphorylatable Spc110S11AS36A were sensitive to the microtubule depolymerizing drug benomyl. In an undisturbed cell cycle, Spc110S11AS36A cells had normal spindles and cell cycle progression until late anaphase. However, Spc110S11AS36A cells were delayed in late-anaphase and presented elevated levels of Cdc14, a protein phosphatase required for mitotic exit, at the SPBs. Thus, we propose that the S11 and S36 phosphosites of Spc110 regulate Cdc14 localization at the SPBs for timely mitotic exit.
HostingRepositoryjPOST
AnnounceDate2023-04-03
AnnouncementXMLSubmission_2023-04-02_20:24:02.003.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRadoslaw Sobota
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; unknown modification; unknown modification
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-07 06:03:28ID requested
12023-04-02 20:24:03announced
Publication List
Abbasi M, Julner A, Lim YT, Zhao T, Sobota RM, Men, é, ndez-Benito V, Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit. Biol Open, 11(11):(2022) [pubmed]
Keyword List
submitter keyword: microtubule
mitosis
spindle
phosphorylation
spindle pole body
pericentrin, tubulin, Cdc14, MEN, yeast.
Contact List
Victoria Menéndez-Benito
lab head
Radoslaw Sobota
contact affiliationIMCB A-STAR Singapore
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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