PXD033067

PXD033067 is an original dataset announced via ProteomeXchange.

Title	Mapping subcellular localizations of unannotated microproteins with MicroID
Description	Proteogenomic and ribosome profiling identification of translated small open reading frames have revealed thousands of microproteins, or polypeptides of less than 100 amino acids, that were previously invisible to geneticists. However, the vast majority of microproteins remain uncharacterized in molecular detail, in part because their lack of conservation and short lengths preclude analysis of homology to protein domains of known function. Proximity-dependent biotinylation technique provided an alternative approach to define the composition of subcellular compartments in living cells and animals. Here, we developed the first high-throughput technology for global mapping of cryptic microproteins to specific subcellular localizations and organelles by proximity-dependent biotinylation technology, thereby coupling their discovery to a dimension of biological functional information. In this work, we showed that hundreds of microproteins are subnuclear organelles associated with critical cellular and biological functions. Moreover, one of these novel microproteins, alt-LAMA3, localized in the nucleolus, is functionally associated with pre-rRNA transcription and required for global protein synthesis during ribosome biogenesis. Lastly, we applied this technique to mouse models, demonstrating the foundation for its use in discovery and characterization of microproteins in animals.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:38.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZHENKUN NA
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:33:19	ID requested
1	2022-08-01 08:14:47	announced
⏵ 2	2023-11-14 08:55:39	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Microprotein

Proximity-dependent biotinylation

Sarah Slavoff
contact affiliation	Yale University
contact email	sarah.slavoff@yale.edu
lab head
ZHENKUN NA
contact affiliation	Yale University
contact email	zhenkun.na@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033067

PRIDE project URI

• PRIDE
  1. PXD033067
     1. Label: PRIDE project
     2. Name: Mapping subcellular localizations of unannotated microproteins with MicroID