⮝ Full datasets listing

PXD033065

PXD033065 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
DescriptionPost-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry for the simultaneous enrichment and analysis of protein N-glycosylation and phosphorylation in pancreas tissues.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_00:33:33.823.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDylan Nicholas Tabang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-07 03:30:22ID requested
12022-08-12 00:33:34announced
Publication List
Tabang DN, Wang D, Li L, A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues. J Vis Exp, (183):(2022) [pubmed]
Keyword List
submitter keyword: simultaneous enrichment, post-translational modifications (PTMs), N-glycopeptides, phosphopeptides, dual-functional Ti(IV)-immobilized metal affinity chromatography (IMAC), electrostatic repulsion hydrophilic interaction chromatography (ERLIC), pancreas
Contact List
Lingjun Li
contact affiliationSchool of Pharmacy, University of Wisconsin-Madison
contact emaillingjun.li@wisc.edu
lab head
Dylan Nicholas Tabang
contact affiliationUniversity of Wisconsin-Madison
contact emailtabang@wisc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033065
PRIDE project URI
Repository Record List
[ + ]