PXD033065 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues |
Description | Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry for the simultaneous enrichment and analysis of protein N-glycosylation and phosphorylation in pancreas tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-12_00:33:33.823.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dylan Nicholas Tabang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-07 03:30:22 | ID requested | |
⏵ 1 | 2022-08-12 00:33:34 | announced | |
Publication List
Tabang DN, Wang D, Li L, A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues. J Vis Exp, (183):(2022) [pubmed] |
Keyword List
submitter keyword: simultaneous enrichment, post-translational modifications (PTMs), N-glycopeptides, phosphopeptides, dual-functional Ti(IV)-immobilized metal affinity chromatography (IMAC), electrostatic repulsion hydrophilic interaction chromatography (ERLIC), pancreas |
Contact List
Lingjun Li |
contact affiliation | School of Pharmacy, University of Wisconsin-Madison |
contact email | lingjun.li@wisc.edu |
lab head | |
Dylan Nicholas Tabang |
contact affiliation | University of Wisconsin-Madison |
contact email | tabang@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033065 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033065
- Label: PRIDE project
- Name: A Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues