PXD033065

PXD033065 is an original dataset announced via ProteomeXchange.

Title	A Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Description	Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry for the simultaneous enrichment and analysis of protein N-glycosylation and phosphorylation in pancreas tissues.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:33:33.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dylan Nicholas Tabang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:30:22	ID requested
⏵ 1	2022-08-12 00:33:34	announced

Tabang DN, Wang D, Li L, A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues. J Vis Exp, (183):(2022) [pubmed]

submitter keyword: simultaneous enrichment, post-translational modifications (PTMs), N-glycopeptides, phosphopeptides, dual-functional Ti(IV)-immobilized metal affinity chromatography (IMAC), electrostatic repulsion hydrophilic interaction chromatography (ERLIC), pancreas

Lingjun Li
contact affiliation	School of Pharmacy, University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Dylan Nicholas Tabang
contact affiliation	University of Wisconsin-Madison
contact email	tabang@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033065
     1. Label: PRIDE project
     2. Name: A Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues