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PXD033061

PXD033061 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp
DescriptionThe freshwater fish crucian carp (Carassius carassius) are able to survive chronic anoxia for several months at low temperatures. Consequently, anoxia-related physiological and biochemical adaptations in this species have been studied for more than half a century. Still, despite for the well-known role of protein phosphorylation in regulating cellular processes, no studies have comprehensively characterized the phosphoproteome in crucian carp. In this study, we report the global phosphoproteome in crucian carp brain and liver during anoxia and reoxygenation. By applying a bottom-up proteomic approach on enriched phosphopeptides we found that the brain phosphoproteome show surprisingly few changes during anoxia-reoxygenation exposure with 110 out of 4316 phosphopeptides being differentially regulated. By contrast, in the liver 395 out of 1293 phosphopeptides were regulated. Although most changes occurred in the liver phosphoproteome, we found evidence for metabolic depression and decreased translation in both brain and liver. We also found regulated phosphoproteins involved in apoptotic regulation and reactive oxygen species handling in both tissues. In the brain, some of the most regulated phosphopeptides belonged to proteins involved in central nervous system development and neuronal activity at the synaptic cleft. Regulated phosphoproteins specific for liver tissue were related to glucose metabolism, including glycolytic flux and glycogenolysis, and the ubiquitin-proteasome system.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:43:14.647.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD033061
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBernd Thiede
SpeciesList scientific name: Carassius carassius; NCBI TaxID: 217509;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-07 03:28:45ID requested
12024-05-29 11:28:14announced
22024-10-22 06:43:15announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD033061;
10.3389/FPHYS.2024.1407834;
Keyword List
submitter keyword: anoxia, crucian carp,Phosphoproteomics
Contact List
Anette Johansen
contact affiliationUniversity of Oslo, Department of Biosciences
contact emailanette.johansen@medisin.uio.no
lab head
Bernd Thiede
contact affiliationUniversity of Oslo
contact emailbernd.thiede@ibv.uio.no
dataset submitter
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Dataset FTP location
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Repository Record List
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