PXD033061 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp |
Description | The freshwater fish crucian carp (Carassius carassius) are able to survive chronic anoxia for several months at low temperatures. Consequently, anoxia-related physiological and biochemical adaptations in this species have been studied for more than half a century. Still, despite for the well-known role of protein phosphorylation in regulating cellular processes, no studies have comprehensively characterized the phosphoproteome in crucian carp. In this study, we report the global phosphoproteome in crucian carp brain and liver during anoxia and reoxygenation. By applying a bottom-up proteomic approach on enriched phosphopeptides we found that the brain phosphoproteome show surprisingly few changes during anoxia-reoxygenation exposure with 110 out of 4316 phosphopeptides being differentially regulated. By contrast, in the liver 395 out of 1293 phosphopeptides were regulated. Although most changes occurred in the liver phosphoproteome, we found evidence for metabolic depression and decreased translation in both brain and liver. We also found regulated phosphoproteins involved in apoptotic regulation and reactive oxygen species handling in both tissues. In the brain, some of the most regulated phosphopeptides belonged to proteins involved in central nervous system development and neuronal activity at the synaptic cleft. Regulated phosphoproteins specific for liver tissue were related to glucose metabolism, including glycolytic flux and glycogenolysis, and the ubiquitin-proteasome system. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:43:14.647.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD033061 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Bernd Thiede |
SpeciesList | scientific name: Carassius carassius; NCBI TaxID: 217509; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-07 03:28:45 | ID requested | |
1 | 2024-05-29 11:28:14 | announced | |
⏵ 2 | 2024-10-22 06:43:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: anoxia, crucian carp,Phosphoproteomics |
Contact List
Anette Johansen |
contact affiliation | University of Oslo, Department of Biosciences |
contact email | anette.johansen@medisin.uio.no |
lab head | |
Bernd Thiede |
contact affiliation | University of Oslo |
contact email | bernd.thiede@ibv.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD033061 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033061
- Label: PRIDE project
- Name: Quantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp