PXD033061

PXD033061 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp
Description	The freshwater fish crucian carp (Carassius carassius) are able to survive chronic anoxia for several months at low temperatures. Consequently, anoxia-related physiological and biochemical adaptations in this species have been studied for more than half a century. Still, despite for the well-known role of protein phosphorylation in regulating cellular processes, no studies have comprehensively characterized the phosphoproteome in crucian carp. In this study, we report the global phosphoproteome in crucian carp brain and liver during anoxia and reoxygenation. By applying a bottom-up proteomic approach on enriched phosphopeptides we found that the brain phosphoproteome show surprisingly few changes during anoxia-reoxygenation exposure with 110 out of 4316 phosphopeptides being differentially regulated. By contrast, in the liver 395 out of 1293 phosphopeptides were regulated. Although most changes occurred in the liver phosphoproteome, we found evidence for metabolic depression and decreased translation in both brain and liver. We also found regulated phosphoproteins involved in apoptotic regulation and reactive oxygen species handling in both tissues. In the brain, some of the most regulated phosphopeptides belonged to proteins involved in central nervous system development and neuronal activity at the synaptic cleft. Regulated phosphoproteins specific for liver tissue were related to glucose metabolism, including glycolytic flux and glycogenolysis, and the ubiquitin-proteasome system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:14.647.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033061
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bernd Thiede
SpeciesList	scientific name: Carassius carassius; NCBI TaxID: 217509;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:28:45	ID requested
1	2024-05-29 11:28:14	announced
⏵ 2	2024-10-22 06:43:15	announced	2024-10-22: Updated project metadata.

10.6019/PXD033061;

10.3389/FPHYS.2024.1407834;

submitter keyword: anoxia, crucian carp,Phosphoproteomics

Anette Johansen
contact affiliation	University of Oslo, Department of Biosciences
contact email	anette.johansen@medisin.uio.no
lab head
Bernd Thiede
contact affiliation	University of Oslo
contact email	bernd.thiede@ibv.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033061
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp