PXD033056

PXD033056 is an original dataset announced via ProteomeXchange.

Title	Proteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways
Description	To simplify chemical risk assessment, the adverse outcome pathway (AOP) method has arisen as a framework to predict the impact of chemical exposure on human and environmental health. The development of this predictive tool requires knowledge of the molecular interaction between chemicals and protein targets. Here, we demonstrate that the identification and selection of a target candidate to develop adverse outcome pathways can be obtained by applying high-throughput identification of chemical targets by proteome integral solubility alteration (PISA) assay, followed by selecting the priority target candidate by an analytical hierarchy process. From 8 identified protein targets for TCDD from a soluble proteome extracted from hepatocytes, we selected the priority target for developing AOPs. Our combined methodologies provide identification of a molecular initiating event, essential knowledge to link molecular interaction to impact on health. The identification of chemical targets following a systematic analysis of chemical protein interaction by proteomics increases the high-throughput and reduces the traditional bias. The target selection eliminates conflicting criteria by applying a multiple-criteria decision-making analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:38.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronica Lizano-Fallas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:11:38	ID requested
1	2023-03-11 10:51:54	announced
⏵ 2	2023-11-14 08:43:42	announced	2023-11-14: Updated project metadata.

Lizano-Fallas V, Carrasco Del Amor A, Cristobal S, Prediction of Molecular Initiating Events for Adverse Outcome Pathways Using High-Throughput Identification of Chemical Targets. Toxics, 11(2):(2023) [pubmed]

submitter keyword: hepatocytes, bioactive compound, pollutants,Proteome integral solubility alteration assay, TCDD, analytical hierarchy process, HepG2

Susana Cristobal
contact affiliation	Department of Biomedical and Clinical Sciences, Cell Biology, Medical Faculty, Linköping University. Sweden
contact email	susana.cristobal@liu.se
lab head
Veronica Lizano-Fallas
contact affiliation	Linköping University
contact email	veronica.lizano@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033056

PRIDE project URI

• PRIDE
  1. PXD033056
     1. Label: PRIDE project
     2. Name: Proteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways