PXD033050

PXD033050 is an original dataset announced via ProteomeXchange.

Title	Compound interaction screen on a photoactivatable cellulose membrane (CISCM) identifies drug targets
Description	Here, we present a compound interaction screen on a photoactivatable cellulose membrane (CISCM) that enables target identification of several drugs in parallel. To this end, we use diazirine-based undirected photoaffinity labeling (PAL) to immobilize compounds on cellulose membranes. Functionalized membranes are then incubated with protein extract and specific targets are identified via quantitative affinity purification and mass spectrometry. CISCM reliably identifies known targets of natural products in less than three hours of analysis time per compound. In summary, we show that combining undirected photoimmobilization of compounds on cellulose with quantitative interaction proteomics provides an efficient means to identify the targets of natural products.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:39:33.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fiona Teresa Melder
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:04:11	ID requested
1	2022-10-25 09:13:41	announced
⏵ 2	2023-11-14 07:39:34	announced	2023-11-14: Updated project metadata.

Melder FTI, Lindemann P, Welle A, Trouillet V, Hei, ß, ler S, Nazar, é M, Selbach M, Compound Interaction Screen on a Photoactivatable Cellulose Membrane (CISCM) Identifies Drug Targets. ChemMedChem, 17(19):e202200346(2022) [pubmed]

submitter keyword: human affinity purification-mass spectrometry samples

Matthias Selbach
contact affiliation	Proteome Dynamics Lab, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
contact email	matthias.selbach@mdc-berlin.de
lab head
Fiona Teresa Melder
contact affiliation	Max Delbrueck Center for Molecular Medicine in the Helmholtz Association
contact email	teresa.melder@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033050

PRIDE project URI

• PRIDE
  1. PXD033050
     1. Label: PRIDE project
     2. Name: Compound interaction screen on a photoactivatable cellulose membrane (CISCM) identifies drug targets