PXD033021

PXD033021 is an original dataset announced via ProteomeXchange.

Title	Reduced mitochondria provide an essential function for the cytosolic methionine cycle
Description	Since the discovery of eukaryotes without mitochondria, intestinal flagellates oxymonads, it was clear that the loss of mitochondria was facilitated by the remodelling of the FeS cluster synthesis. Yet, this remodelling is common for a broader linage including free-living species with reduced mitochondria and so question remains what the functions of their organelles are. We resolved to a high precision the proteome of one of them using Localisation of Organelle Proteins by Isotope Tagging (LOPIT) and demonstrate that it performs the synthesis of folate derivates bearing one carbon units. This pathway links to a previously localised glycine cleavage system (GCS) and its only conceivable client seems cytosolic methionine cycle recycling S-adenosine methionine. This observation gives a sense for the consistent presence of GCS in reduced mitochondria of free-living anaerobes and its absence in endobionts, which typically lose the methionine cycle and in the case of oxymonads also mitochondria.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:13.503.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Paratrimastix pyriformis; NCBI TaxID: 342808;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-06 08:54:23	ID requested
1	2023-03-11 06:53:27	announced
⏵ 2	2023-11-14 08:43:21	announced	2023-11-14: Updated project metadata.

Z, í, tek J, F, ü, ssy Z, Treitli SC, Pe, ñ, a-Diaz P, Vaitov, á Z, Zavadska D, Harant K, Hampl V, Reduced mitochondria provide an essential function for the cytosolic methionine cycle. Curr Biol, 32(23):5057-5068.e5(2022) [pubmed]

submitter keyword: Reduced mitochondria, glycine cleavage system, Paratrimastix, mitochondrial loss, LOPIT, one-carbon metabolism, proteome, methionine cycle

Vladimír Hampl
contact affiliation	Charles University, Faculty of Science, Department of Parasitology, BIOCEV, Vestec 252 50, Czech Republic.
contact email	vladimir.hampl@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033021

PRIDE project URI

• PRIDE
  1. PXD033021
     1. Label: PRIDE project
     2. Name: Reduced mitochondria provide an essential function for the cytosolic methionine cycle