PXD033018

PXD033018 is an original dataset announced via ProteomeXchange.

Title	Regulation of mRNA localization during the oocyte-to-embryo transition by regulated mRNA-endoplasmic reticulum association and remodeling of the endoplasmic reticulum
Description	As an important post-transcriptional regulatory mechanism, asymmetric localization of mRNAs is essential for cell polarity and cell fate determination during early development. In an effort to understand localization of mRNAs encoding proteasome components during Xenopus oocyte-to-embryo transition, we discovered that the endoplasmic reticulum (ER), which is thought to mainly serve “house-keeping” roles in the cell, plays a multifaceted role in controlling localization of maternal RNAs. Our RNA-seq analysis of fractionated transcripts reveals that more than 40% of RNAs, including proteasome mRNAs, are present on the ER in Xenopus oocytes. After meiotic maturation, a large fraction of ER-associated RNA is released into the cytosol. The release of proteasome RNAs from the ER into the cytosol is conserved during mouse oocyte maturation. Our comparative proteomic analysis and ribonucleoprotein immunoprecipitation demonstrate that the majority of ER-associated RNA-binding proteins (RBPs) remain associated with the ER after oocyte maturation. However, all ER-associated RBPs analyzed exhibit reduced binding to some of their target RNAs after oocyte maturation, providing a mechanistic explanation for the dynamic regulation of RNA-ER association. We further show that the ER is remodeled massively during Xenopus oocyte maturation, leading to the formation of a widespread tubular ER network in the animal hemisphere that is required for the asymmetric localization of proteasome mRNAs in mature eggs. To our knowledge, our findings demonstrate for the first time that dynamic regulation of RNA-ER association and remodeling of the ER are fundamentally important for asymmetric localization of RNAs during development.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:01.924.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033018
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hyojeong Hwang
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-06 08:28:48	ID requested
1	2022-12-15 13:13:34	announced
⏵ 2	2023-11-14 08:55:02	announced	2023-11-14: Updated project metadata.

10.6019/PXD033018;

submitter keyword: Xenopus laevis, microsome. oocyte maturation

Jing Yang
contact affiliation	Department of Comparative Biosciences, University of Illinois at Urbana-Champaign
contact email	yangj@illinois.edu
lab head
Hyojeong Hwang
contact affiliation	University of Illinois Urbana-Champaign
contact email	hh8@illinois.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD033018

PRIDE project URI

• PRIDE
  1. PXD033018
     1. Label: PRIDE project
     2. Name: Regulation of mRNA localization during the oocyte-to-embryo transition by regulated mRNA-endoplasmic reticulum association and remodeling of the endoplasmic reticulum