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PXD032968

PXD032968 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes
DescriptionNicotinamide adenine dinucleotide phosphate (NADPH) is the primary electron donor for reductive reactions that are essential for the biosynthesis of major cell components in all organisms. Nicotinamide adenine dinucleotide kinase (NADK) is the only enzyme that catalyzes synthesis of NADP(H) from NAD(H). While the enzymatic properties and physiological functions of NADK have been thoroughly studied, the role of NADK in bacterial pathogenesis remains unknown. Here, we used CRISPR interference to knockdown NADK gene expression to address the role of this enzyme in Staphylococcus aureus pathogenic potential. We find that NADK inhibition drastically decreases mortality of zebrafish infected with S. aureus. Further, we show that NADK promotes S. aureus survival in infected macrophages by protecting bacteria from antimicrobial defense mechanisms. Proteome-wide data analysis revealed that production of major virulence associated factors is sustained by NADK. We demonstrate that NADK is required for expression of the quorum-sensing response regulator AgrA, which controls critical S. aureus virulence determinants. These findings support a key role for NADK in bacteria survival within innate immune cells and the host during infection.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_00:40:22.671.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMagalie Duchateau
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-03 07:35:57ID requested
12022-08-12 00:40:23announced
Publication List
Leseigneur C, Boucontet L, Duchateau M, Pizarro-Cerda J, Matondo M, Colucci-Guyon E, Dussurget O, pathogenesis by supporting production of virulence factors and protective enzymes. Elife, 11():(2022) [pubmed]
Keyword List
submitter keyword: Nicotinamide adenine dinucleotide kinase,Staphylococcus aureus ,
Contact List
Olivier Dussurget
contact affiliationYersinia Research Unit Duclaux Building, Bertrand wing – Ground Floor Institut Pasteur, 28, rue du Docteur Roux 75015 Paris
contact emailolivier.dussurget@pasteur.fr
lab head
Magalie Duchateau
contact affiliationInstitut Pasteur
contact emailmagalie.duchateau@pasteur.fr
dataset submitter
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Dataset FTP location
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