PXD032962

PXD032962 is an original dataset announced via ProteomeXchange.

Title	Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Squamous Cell Carcinoma Subtype
Description	Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients, exactly squamous cell carcinoma (SCC), taken from the tumor center and tumor margin and control (adjacent non-tumor) tissues. We found and 21 spots representing 20 proteins differentiating center and margin. We found 111 differentially expressed protein spots representing 95 proteins; 84, 7, and 20 spots differed between the control and center and margin, control and center, and control and margin, respectively. Nine significant canonical pathways were identified, including hypoxia-inducible factor-1α signaling, thyroid hormone biosynthesis, and phagosome maturation. Several pathways related to disease and function were identified as related to cell death and survival, and several related to cancer, organismal injury, and abnormalities. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, epithelialmesenchymal transition, epithelial adherens junctions, and inflammatory responses.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_08:53:25.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Dietrich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-03 07:30:08	ID requested
⏵ 1	2022-05-20 08:53:27	announced

Ciereszko A, Dietrich MA, S, ł, owi, ń, ska M, Nynca J, Ciborowski M, Kaczmarek MM, Myszczy, ń, ski K, Ki, ś, luk J, Majewska A, Michalska-Falkowska A, Kodzik N, Resze, ć J, Sierko E, Nikli, ń, ski J, Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype. PLoS One, 17(5):e0268073(2022) [pubmed]

submitter keyword: human, tissue, lung, cancer, SCC, squamous cell carcinoma

Mariola Dietrich
contact affiliation	Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences
contact email	m.dietrich@pan.olsztyn.pl
lab head
Mariola Dietrich
contact affiliation	Polish Academy of Sciences
contact email	m.dietrich@pan.olsztyn.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032962
     1. Label: PRIDE project
     2. Name: Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Squamous Cell Carcinoma Subtype