PXD032959

PXD032959 is an original dataset announced via ProteomeXchange.

Title	Tic20-like proteins mediate organelle assembly in Alphaproteobacteria
Description	Organelle-specific protein translocation systems are essential for the biogenesis of many organelles in eukaryotes but thought to be absent from prokaryotic organelles like magnetosomes. Here, using evolutionary analyses, we show that magnetosomal MamF-like proteins represent members of the widespread yet uncharacterized DUF4870 family that shares an ancient evolutionary origin with plastidal Tic20 preprotein translocases. Deletion of mamF-like genes in Magnetospirillum gryphiswaldense results in magnetosome misassembly and thereby impairs magnetite biomineralization, magnetosome chain formation, and magnetotaxis. While soluble in their absence, misassembled proteins like MamJ interact with MamF-like proteins via glycine-rich C-terminal transmembrane domains. Magnetosome membrane complexes of MamJ, however, are carbonate-resistant and largely dissociated from MamF-like proteins suggesting that MamF-like proteins mediate organelle-specific membrane protein insertion. The DUF4870 family was therefore renamed into MART family (membrane assembly by remote Tic20 homologs). Collectively, our data indicate a conserved role for the Tic20/MART superfamily in protein trafficking across all domains of life.
HostingRepository	PRIDE
AnnounceDate	2024-11-19
AnnouncementXML	Submission_2024-11-19_11:16:45.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Sura
SpeciesList	scientific name: Magnetospirillum gryphiswaldense; NCBI TaxID: 55518;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-03 07:23:27	ID requested
⏵ 1	2024-11-19 11:16:46	announced

Dataset with its publication pending

submitter keyword: MART,magnetosomes, organelles, protein translocation, MamJ, Tic20, DUF4870, magnetokeleton

Doerte Becher
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics
contact email	doerte.becher@uni-greifswald.de
lab head
Thomas Sura
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics
contact email	thomas.sura@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032959
     1. Label: PRIDE project
     2. Name: Tic20-like proteins mediate organelle assembly in Alphaproteobacteria